1887

Abstract

A xylanolytic bacterium, strain S1-3, was isolated from soil collected in Nan province, Thailand. It was characterized taxonomically based on phenotypic characteristics and 16S rRNA gene sequence comparison. The strain was a Gram-stain-positive, facultatively anaerobic, spore-forming, rod-shaped bacterium. It contained -diaminopimelic acid in the cell-wall peptidoglycan. The major menaquinone was MK-7. Iso-C (39.5 %) and anteiso-C (26.8 %) were predominant cellular fatty acids. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and lysyl-phosphatidylglycerol were the major polar lipids. The DNA G+C content was 53.3 mol%. Phylogenetic analysis using 16S rRNA gene sequences showed that strain S1-3 was affiliated to the genus , and was closely related to GR21-5 and CCUG 47242 with 95.7 and 95.3 % sequence similarity, respectively. Strain S1-3 could be clearly distinguished from related species of the genus by its physiological and biochemical characteristics as well as by its phylogenetic position. Therefore, the strain represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is S1-3 (=KCTC 22296 =TISTR 1890 =PCU 306).

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2010-10-01
2020-01-19
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Polar lipid profile of strain S1-3 after separation by two-dimensional thin layer chromatography. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; LPG, lysyl-phosphatidylglycerol; PL1, unknown phospholipid; PN1–3, unknown aminophospholipids.

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