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Volume 60, Issue 7

gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of as comb. nov. Free

Abstract

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (Sa25) was isolated from air of a duck barn. 16S rRNA gene and sequence analyses clearly placed the isolate in the vicinity of the –– group, with the closest relative being KMM 3858. This allocation was confirmed by analyses of the quinone system (ubiquinone Q-10), fatty acid data (major fatty acids C 7 and C cyclo 8) and polar lipid profile (major components diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unknown aminolipid AL1; moderate amounts of three unknown polar lipids, L1–L3, an unknown aminolipid and an unknown aminophospholipid APL2). The polyamine pattern of Sa25 exhibited the major compound putrescine and moderate amounts of spermidine; a similar polyamine pattern with the major compound putrescine was also detected in KMM 3858. DNA–DNA hybridization of strain Sa25 with KMM 3858 and the type strains of the other species showed values ranging from 50.3 to 24.8 %, and physiological and biochemical data clearly differentiated this isolate from the described species. Since Sa25 and KMM 3858 form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, and this separate position is supported by unique characteristics of their polyamine patterns and polar lipid profiles, we propose the novel genus gen. nov., with the type species sp. nov. (type strain Sa25 =CCUG 57736 =CCM 7656) and the reclassification of as comb. nov. (type strain Pi26 =KMM 3858 =NRIC 0733 =JCM 15115).

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2010-07-01
2024-10-09
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
     [Google Scholar]
  2. Busse H.-J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
     [Google Scholar]
  3. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [CrossRef]
     [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
     [Google Scholar]
  6. Huber B., Scholz H. C., Kämpfer P., Falsen E., Langer S., Busse H.-J. 2010; Ochrobactrum pituitosum sp. nov., isolated from an industrial environment. Int J Syst Evol Microbiol 60:321–326 [CrossRef]
     [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
     [Google Scholar]
  8. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
     [Google Scholar]
  9. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
     [Google Scholar]
  10. Kämpfer P., Rosselló-Mora R., Scholz H., Welinder-Olsen C., Busse H.-J. 2006; Description of Pseudochrobactrum gen. nov., with the two species Pseudochrobactrum asaccharolyticum sp.nov. and Pseudochrobactrumsaccharolyticum sp. nov. Int J Syst Evol Microbiol 56:1823–1829 [CrossRef]
     [Google Scholar]
  11. Kämpfer P., Scholz H., Huber B., Thummes K., Busse H.-J., Maas E. W., Falsen E. 2007a; Description of Pseudochrobactrum kiredjianiae sp. nov. Int J Syst Evol Microbiol 57:755–760 [CrossRef]
     [Google Scholar]
  12. Kämpfer P., Scholz H. C., Falsen E., Busse H.-J. 2007b; Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 57:2513–2518 [CrossRef]
     [Google Scholar]
  13. Kämpfer P., Sessitsch A., Schloter M., Huber B., Busse H.-J., Scholz H. C. 2008; Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environment. Int J Syst Evol Microbiol 58:1426–1431 [CrossRef]
     [Google Scholar]
  14. Kämpfer P., Huber B., Lodders N., Warfolomeow I., Busse H.-J., Scholz H. C. 2009; Pseudochrobactrum lubricantis sp. nov., isolated from a metal-working fluid. Int J Syst Evol Microbiol 59:2464–2467 [CrossRef]
     [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
     [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  18. Olsen G. J., Matsuda H., Hagström R., Overbeek R. 1994; fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10:41–48
     [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35:7188–7196 [CrossRef]
     [Google Scholar]
  20. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2008; Pseudochrobactrum glaciei sp. nov., isolated from sea ice collected from Peter the Great Bay of the Sea of Japan. Int J Syst Evol Microbiol 58:2454–2458 [CrossRef]
     [Google Scholar]
  21. Scholz H. C., Tomaso H., Al Dahouk S., Witte A., Schloter M., Kämpfer P., Falsen E., Neubauer H. 2006; Genotyping of Ochrobactrum anthropi by recA -based comparative sequence, PCR-RFLP, and 16S rRNA gene analysis. FEMS Microbiol Lett 257:7–16 [CrossRef]
     [Google Scholar]
  22. Scholz H. C., Hubalek Z., Sedláček I., Vergnaud G., Tomaso H., Al Dahouk S., Melzer F., Kämpfer P., Neubauer H. other authors 2008; Brucella microti sp. nov., isolated from the common vole Microtus arvalis . Int J Syst Evol Microbiol 58:375–382 [CrossRef]
     [Google Scholar]
  23. Scholz H. C., Nöckler K., Göllner C., Bahn P., Vergnaud G., Tomaso H., Al Dahouk S., Kämpfer P., Cloeckaert A. other authors 2010; Brucella inopinata sp. nov., isolated from a breast implant infection. Int J Syst Evol Microbiol 60:801–808 [CrossRef]
     [Google Scholar]
  24. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [CrossRef]
     [Google Scholar]
  25. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
     [Google Scholar]
  26. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
     [Google Scholar]
  27. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
     [Google Scholar]
  28. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
     [Google Scholar]
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Paenochrobactrum gallinarii gen. nov., sp. nov., isolated from air of a duck barn, and reclassification of Pseudochrobactrum glaciei as Paenochrobactrum glaciei comb. nov.
Int J Syst Evol Microbiol 60, 1493 (2010); https://doi.org/10.1099/ijs.0.015842-0
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