1887

Abstract

A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (Sa25) was isolated from air of a duck barn. 16S rRNA gene and sequence analyses clearly placed the isolate in the vicinity of the group, with the closest relative being KMM 3858. This allocation was confirmed by analyses of the quinone system (ubiquinone Q-10), fatty acid data (major fatty acids C 7 and C cyclo 8) and polar lipid profile (major components diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and unknown aminolipid AL1; moderate amounts of three unknown polar lipids, L1–L3, an unknown aminolipid and an unknown aminophospholipid APL2). The polyamine pattern of Sa25 exhibited the major compound putrescine and moderate amounts of spermidine; a similar polyamine pattern with the major compound putrescine was also detected in KMM 3858. DNA–DNA hybridization of strain Sa25 with KMM 3858 and the type strains of the other species showed values ranging from 50.3 to 24.8 %, and physiological and biochemical data clearly differentiated this isolate from the described species. Since Sa25 and KMM 3858 form a distinct lineage in the 16S rRNA gene sequence-based phylogenetic tree, and this separate position is supported by unique characteristics of their polyamine patterns and polar lipid profiles, we propose the novel genus gen. nov., with the type species sp. nov. (type strain Sa25 =CCUG 57736 =CCM 7656) and the reclassification of as comb. nov. (type strain Pi26 =KMM 3858 =NRIC 0733 =JCM 15115).

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2010-07-01
2019-12-11
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Phylogenetic tree reconstruction with sequences (909 nt) using CLUSTREE neighbour-joining analysis. [PDF](333 KB)

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Total polar lipid profile of Sa25 after two-dimensional TLC and detection with molybdatophosphoric acid. The first dimension is horizontal. PME, Phosphatidylmonomethylethanolamine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; AL1-3, unknown aminolipids; APL1-APL2, unknown aminophospholipids; L1-5, unknown polar lipids; pPigm, pink pigment.

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Major fatty acids (%) of strain Sa25 , comb. nov. KMM 3858 and type strains of species. [PDF](41 KB)

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