1887

Abstract

The taxonomic status of an actinomycete isolated from soil collected from a hay meadow was determined using a polyphasic approach. The strain, designated N1350, had morphological and chemotaxonomic properties consistent with its classification in the genus and formed a distinct phyletic line within the clade comprising the type strains of species of the genus in the 16S rRNA gene tree. Strain N1350 shared highest 16S rRNA gene sequence similarities with MT8 (98.1 %) and MA140-96 (98.3 %). However, strain N1350 was readily distinguished from the type strains of species using a combination of phenotypic properties. On the basis of these data, strain N1350 is considered to represent a novel species of the genus The name proposed for this taxon is sp. nov., with the type strain N1350 (=DSM 45372 =NCIMB 14575 =NRRL B-24794).

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2010-11-01
2019-10-20
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References

  1. Adachi, K., Katsuta, A., Matsuda, S., Peng, X., Misawa, N., Shizuri, Y., Kroppenstedt, R. M., Yokota, A. & Kasai, H. ( 2007; ). Smaragdicoccus niigatensis gen. nov., sp. nov., a novel member of the suborder Corynebacterineae. Int J Syst Evol Microbiol 57, 297–301.[CrossRef]
    [Google Scholar]
  2. Atalan, E., Manfio, G. P., Ward, A. C., Kroppenstedt, R. M. & Goodfellow, M. ( 2000; ). Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77, 337–353.[CrossRef]
    [Google Scholar]
  3. Collins, M. D. ( 1994; ). Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by Goodfellow, M. & O'Donnell, A. G.. Chichester. : Wiley.
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  8. Goodfellow, M. & Maldonado, L. A. ( 2006; ). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 843–888. Edited by Dworkin, M., Falkow, S., Rosenberg, E., Schleifer, K. H. & Stackebrandt, E.. New York. : Springer.
    [Google Scholar]
  9. Gordon, R. E. & Mihm, J. M. ( 1962; ). Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98, 628–636.
    [Google Scholar]
  10. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  11. Jones, A. L., Koerner, R. J., Natarajan, S., Perry, J. D. & Goodfellow, M. ( 2008; ). Dietzia papillomatosis sp. nov., a novel actinomycete isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis. Int J Syst Evol Microbiol 58, 68–72.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro, H. N.. New York. : Academic Press.
    [Google Scholar]
  13. Kämpfer, P., Andersson, M. A., Rainey, F. A., Kroppenstedt, R. M. & Salkinoja-Salonen, M. ( 1999; ). Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children's day care centre. Int J Syst Bacteriol 49, 681–687.[CrossRef]
    [Google Scholar]
  14. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M. ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef]
    [Google Scholar]
  15. Kluge, A. G. & Farris, J. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  16. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow, M. & Minnikin, D. E.. New York. : Academic Press.
    [Google Scholar]
  17. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  18. Minnikin, D. E., Alshamaony, L. & Goodfellow, M. ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  20. Pathom-Aree, W., Nogi, Y., Sutcliffe, I. C., Ward, A. C., Horikoshi, K., Bull, A. T. & Goodfellow, M. ( 2006; ). Williamsia marianensis sp. nov., a novel actinomycete isolated from the Mariana Trench. Int J Syst Evol Microbiol 56, 1123–1126.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Soddell, J. A., Stainsby, F. M., Eales, K. L., Kroppenstedt, R. M., Seviour, R. J. & Goodfellow, M. ( 2006; ). Millisia brevis gen. nov., sp. nov., an actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 56, 739–744.[CrossRef]
    [Google Scholar]
  23. Stach, J. E., Maldonado, L. A., Ward, A. C., Bull, A. T. & Goodfellow, M. ( 2004; ). Williamsia maris sp. nov., a novel actinomycete isolated from the Sea of Japan. Int J Syst Evol Microbiol 54, 191–194.[CrossRef]
    [Google Scholar]
  24. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  25. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  26. Sutcliffe, I. C. ( 2000; ). Characterisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia maris. Antonie van Leeuwenhoek 78, 195–201.[CrossRef]
    [Google Scholar]
  27. Tan, G. Y., Ward, A. C. & Goodfellow, M. ( 2006; ). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Syst Appl Microbiol 29, 557–569.[CrossRef]
    [Google Scholar]
  28. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Yassin, A. F. & Hupfer, H. ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56, 193–197.[CrossRef]
    [Google Scholar]
  31. Yassin, A. F., Young, C. C., Lai, W. A., Hupfer, H., Arun, A. B., Shen, F. T., Rekha, P. D. & Ho, M. J. ( 2007; ). Williamsia serinedens sp. nov., isolated from an oil-contaminated soil. Int J Syst Evol Microbiol 57, 558–561.[CrossRef]
    [Google Scholar]
  32. Zhi, X. Y., Li, W. J. & Stackebrandt, E. ( 2009; ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 59, 589–608.[CrossRef]
    [Google Scholar]
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