1887

Abstract

strain AI-S262 was isolated from the rhizoplane of neem seedlings in the Botanical garden of Tamilnadu Agricultural University, Coimbatore, India, and subjected to phenotypic, chemotaxonomic and genetic characterization. Cells of this strain were Gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. Strain AI-S262 contained MK-12 and MK-13 as the main respiratory quinones, anteiso-C, anteiso-C and iso-C as the predominant fatty acids, peptidoglycan-type B2 with glycolyl residues, and had a DNA G+C content of 69.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed 98.0–98.6 % pair-wise similarity with respect to close relatives in the genus . DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 39%) between strain AI-S262 and its closest relatives. Data from DNA–DNA hybridization and phenotypic analyses supported the conclusion that strain AI-S262 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is AI-S262 (=JCM 15681 =LMG 24772 =KCTC 19668).

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2010-07-01
2019-10-21
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Scanning electron micrograph of cells of strain AI-S262 grown on R2A agar medium after 48h at 28°C. Bar, 1µm.

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