1887

Abstract

A Gram-stain-positive, aerobic actinomycete, strain YIM 60513, was isolated from the stem of L. collected from tropical rainforest at Xishuangbanna, Yunnan Province, south-west China. 16S rRNA gene sequence analysis indicated that strain YIM 60513 belonged to the genus and was closely related to NCIB 12823 (99.1 % similarity) and SPE 10-1 (97.3 % similarity). Data for the predominant quinone [MK-9(H)], major fatty acids (iso-C, anteiso-C and C 9) and G+C content of the genomic DNA (71.6 mol%) were similar to those for members of the genus . The level of DNA–DNA relatedness between strain YIM 60513 and . NCIB 12823 was 43 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic characteristics and DNA–DNA hybridization data supported the view that strain YIM 60513 should be distinguished from . NCIB 12823 and . SPE 10-1. Strain YIM 60513 therefore represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 60513 (=KCTC 19243 =CCTCC AA 207006).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015792-0
2010-05-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/5/1147.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015792-0&mimeType=html&fmt=ahah

References

  1. Araújo, W. L., Marcon, J., Maccheroni, W., Jr, Van Elsas, J. D., Van Vuurde, J. W. & Azevedo, J. L. ( 2002; ). Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants. Appl Environ Microbiol 68, 4906–4914.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Coombs, J. T. & Franco, C. M. ( 2003; ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol 69, 5603–5608.[CrossRef]
    [Google Scholar]
  4. Crawford, D. L., Lynch, J. M., Whipps, J. M. & Ousley, M. A. ( 1993; ). Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl Environ Microbiol 59, 3899–3905.
    [Google Scholar]
  5. Embley, T. M., Wait, R., Dobson, G. & Goodfellow, M. ( 1987; ). Fatty acid composition in the classification of Saccharopolyspora hirsuta. FEMS Microbiol Lett 41, 131–135.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Goodfellow, M., Lacey, J., Athalye, M., Embley, T. M. & Bowen, T. ( 1989; ). Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139.
    [Google Scholar]
  9. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  10. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  11. Gu, Q., Luo, H. L., Zheng, W., Liu, Z. H. & Huang, Y. ( 2006; ). Pseudonocardia oroxyli sp. nov., isolated from Oroxylum indicum root. Int J Syst Evol Microbiol 56, 2193–2197.[CrossRef]
    [Google Scholar]
  12. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  13. He, L., Li, W., Huang, Y., Wang, L., Liu, Z., Lanoot, B., Vancanneyt, M. & Swings, J. ( 2005; ). Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef]
    [Google Scholar]
  14. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  15. Kelly, K. L. ( 1964; ). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  16. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  17. Korn-Wendisch, F., Kempf, A., Grund, E., Kroppenstedt, T. & Kutzner, H. J. ( 1989; ). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.[CrossRef]
    [Google Scholar]
  18. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  19. Labeda, D. P. ( 1987; ). Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.[CrossRef]
    [Google Scholar]
  20. Lacey, J. & Goodfellow, M. ( 1975; ). A novel actinomycete from sugarcane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88, 75–85.[CrossRef]
    [Google Scholar]
  21. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  22. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  23. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  24. Li, J., Zhao, G. Z., Qin, S., Huang, H. Y., Zhu, W. Y., Xu, L. H. & Li, W. J. ( 2009; ). Saccharopolyspora tripterygii sp. nov., an endophytic actinomycete isolated from the stem of Tripterygium hypoglaucum. Int J Syst Evol Microbiol 59, 3040–3044.[CrossRef]
    [Google Scholar]
  25. Lu, Z., Liu, Z., Wang, L., Qi, W. & Goodfellow, M. ( 2001; ). Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325.
    [Google Scholar]
  26. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  27. Mertz, F. P. & Yao, R. C. ( 1990; ). Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40, 34–39.[CrossRef]
    [Google Scholar]
  28. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  29. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef]
    [Google Scholar]
  30. Pimentel-Elardo, S. M., Tiro, L. P., Grozdanov, L. & Hentschel, U. ( 2008; ). Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 58, 628–632.[CrossRef]
    [Google Scholar]
  31. Qin, S., Wang, H.-B., Chen, H.-H., Zhang, Y.-Q., Jiang, C.-L., Xu, L.-H. & Li, W.-J. ( 2008a; ). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. Int J Syst Evol Microbiol 58, 2525–2528.[CrossRef]
    [Google Scholar]
  32. Qin, S., Li, J., Zhao, G. Z., Chen, H. H., Xu, L. H. & Li, W. J. ( 2008b; ). Saccharopolyspora endophytica sp. nov., an endophytic actinomycete isolated from the root of Maytenus austroyunnanensis. Syst Appl Microbiol 31, 352–357.[CrossRef]
    [Google Scholar]
  33. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  34. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  35. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  36. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  37. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 151–194. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  38. Tang, S. K., Wang, Y., Cai, M., Zhi, X. Y., Lou, K., Xu, L. H., Jiang, C. L. & Li, W. J. ( 2009a; ). Saccharopolyspora halophila sp. nov., a novel halophilic actinomycete isolated from a saline lake in China. Int J Syst Evol Microbiol 59, 555–558.[CrossRef]
    [Google Scholar]
  39. Tang, S. K., Wang, Y., Wu, J. Y., Cao, L. L., Lou, K., Xu, L. H., Jiang, C. L. & Li, W. J. ( 2009b; ). Saccharopolyspora qijiaojingensis sp. nov., halophilic actinomycete isolated from a salt lake. Int J Syst Evol Microbiol 59, 2166–2170.[CrossRef]
    [Google Scholar]
  40. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  41. Waksman, S. A. ( 1967; ). The Actinomycetes. A Summary of Current Knowledge. New York: Ronald Press.
  42. Warwick, S. T., Bowen, T., McVeigh, H. & Embley, T. M. ( 1994; ). A phylogenetic analysis of the family Pseudonocardiaceae and the genera Actinokineospora and Saccharothrix with 16S rRNA sequences and a proposal to combine the genera Amycolata and Pseudonocardia in an emended genus Pseudonocardia. Int J Syst Bacteriol 44, 293–299.[CrossRef]
    [Google Scholar]
  43. Yassin, A. F. ( 2009; ). Saccharopolyspora rosea sp. nov., isolated from a patient with bronchial carcinoma. Int J Syst Evol Microbiol 59, 1148–1152.[CrossRef]
    [Google Scholar]
  44. Yuan, L. J., Zhang, Y. Q., Guan, Y., Wei, Y. Z., Li, Q. P., Yu, L. Y., Li, W. J. & Zhang, Y. Q. ( 2008; ). Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58, 1180–1185.[CrossRef]
    [Google Scholar]
  45. Zhang, J. L., Wu, D. D., Zhang, J., Liu, Z. H. & Song, F. ( 2008; ). Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58, 1094–1099.[CrossRef]
    [Google Scholar]
  46. Zhang, J., Wu, D., Liu, Z. & Chao, F. ( 2009; ). Saccharopolyspora jiangxiensis sp. nov., a novel actinobacterium isolated from grass soil. Int J Syst Evol Microbiol 59, 1076–1081.[CrossRef]
    [Google Scholar]
  47. Zhou, Z. H., Liu, Z. H., Qian, Y. D., Kim, S. B. & Goodfellow, M. ( 1998; ). Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015792-0
Loading
/content/journal/ijsem/10.1099/ijs.0.015792-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1147 - 1151

Scanning electron micrograph showing curved chains of smooth-surfaced spores of a 28-day-old culture of strain YIM 60513 grown on ISP medium 2 at 28 °C. Bar, 5 µm.



IMAGE

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error