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Abstract

A Gram-negative, moderately halophilic, non-motile, aerobic bacterium, designated strain YIM D10, was isolated from a salt mine sediment sample from Yunnan, south-west China. The strain grew optimally in the presence of 3–8 % NaCl and at 28 °C and pH 7.5. The polar lipid profile of strain YIM D10 comprised diphosphatidylglycerol, an unknown phospholipid and two unknown aminolipids. The major cellular fatty acids were C 7 (30.5 %), C cyclo 8 (29.3 %) and C (13.2 %). The respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content was 65.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM D10 was closely related to RS.Sph.026 (96.8 % gene sequence similarity). Results confirmed the placement of isolate YIM D10 within the genus . However, DNA–DNA hybridization between strain YIM D10 and the type strain of the only recognized species of the genus , RS.Sph.026, was 16.7 %, showing clearly that the isolate constitutes a new genospecies. Strain YIM D10 could be clearly differentiated from and other phylogenetic neighbours on the basis of some phenotypic, genotypic and chemotaxonomic features. Therefore, strain YIM D10 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YIM D10 (=DSM 21202=CCTCC AA 208035).

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2010-08-01
2019-10-22
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vol. , part 8, pp. 1750 - 1754

Transmission electron micrograph of cells of strain YIM D10 grown for 3 days on TSA containing 3% (w/v) NaCl. Bar, 1µm.



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