1887

Abstract

Thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus (Hymenoptera: Formicidae: tribe Attini). Yeasts isolated from the fungus garden and waste deposit included , , and . In addition, one morphological type was isolated overwhelmingly. Sequencing data of partial large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) region coupled with morphological and physiological characterization accommodated this morphotype in a separate taxonomic position in relation to the known species of (Basidiomycota: Trichosporonales). Here, we propose a novel yeast species named sp. nov. based on the description of 34 isolates; the type strain is strain FCP 540806 (=CBS 11177).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015727-0
2010-06-01
2021-03-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/6/1454.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015727-0&mimeType=html&fmt=ahah

References

  1. Carreiro S. C., Pagnocca F. C., Bueno O. C., Bacci M. Jr, Hebling M. J. A., Silva O. A. 1997; Yeasts associated with nests of the leaf-cutting ant Atta sexdens rubropilosa Forel, 1908. Antonie van Leeuwenhoek 71:243–248 [CrossRef]
    [Google Scholar]
  2. Carreiro S. C., Pagnocca F. C., Bacci M. Jr, Lachance M. A., Bueno O. C., Hebling M. J. A., Ruivo C. C. C., Rosa C. A. 2004; Sympodiomyces attinorum sp. nov., a yeast species associated with nests of the leaf-cutting ant Atta sexdens . Int J Syst Evol Microbiol 54:1891–1894 [CrossRef]
    [Google Scholar]
  3. Craven S. E., Dix M. W., Michaels G. E. 1970; Attine fungus gardens contain yeasts. Science 169:184–186 [CrossRef]
    [Google Scholar]
  4. Currie C. R., Mueller U. G., Malloch D. 1999; The agricultural pathology of ant fungus gardens. Proc Natl Acad Sci U S A 96:7998–8002 [CrossRef]
    [Google Scholar]
  5. Fuentefria A. M., Suh S.-O., Landell M. F., Faganello J., Schrank A., Vainstein M. H., Blackwell M., Valente P. 2008; Trichosporon insectorum sp. nov., a new anamorphic basidiomycetous killer yeast. Mycol Res 112:93–99 [CrossRef]
    [Google Scholar]
  6. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  7. Hölldobler B., Wilson E. O. 1990 The Ants Cambridge, MA: Harvard University Press;
    [Google Scholar]
  8. Inácio J., Rodrigues M. G., Sobral P., Fonseca A. 2004; Characterization and classification of phylloplane yeasts from Portugal related to the genus Taphrina and description of five novel Lalaria species. FEMS Yeast Res 4:541–555 [CrossRef]
    [Google Scholar]
  9. Little A. E. F., Currie C. R. 2007; Symbiont complexity: discovery of a fifth symbiont in the attine ant–microbe symbiosis. Biol Lett 3:501–504 [CrossRef]
    [Google Scholar]
  10. Middelhoven W. J., Fonseca A., Carreiro S. C., Pagnocca F. C., Bueno O. C. 2003; Cryptococcus haglerorum , sp. nov., an anamorphic basidiomycetous yeast isolated from nests of the leaf-cutting ant Atta sexdens . Antonie van Leeuwenhoek 83:167–174 [CrossRef]
    [Google Scholar]
  11. Middelhoven W. J., Scorzetti G., Fell J. W. 2004; Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense , T. smithiae , T. dehoogii ,T. scarabaeorum and T. gamsii . Int J Syst Evol Microbiol 54:975–986 [CrossRef]
    [Google Scholar]
  12. Mueller U. G., Rehner S. A., Schultz T. R. 1998; The evolution of agriculture in ants. Science 281:2034–2038 [CrossRef]
    [Google Scholar]
  13. Mueller U. G., Schultz T. R., Currie C. R., Adams R. M. M., Malloch D. 2001; The origin of the Attini ant–fungus symbiosis. Q Rev Biol 76:169–197 [CrossRef]
    [Google Scholar]
  14. Mueller U. G., Gerardo N. M., Aanen D. K., Six D. L., Schultz T. R. 2005; The evolution of agriculture in insects. Annu Rev Ecol Evol Syst 36:563–595 [CrossRef]
    [Google Scholar]
  15. Nunn M. A., Schafer S. M., Petrou M. A., Brown J. R. M. 2007; Environmental source of Candida dubliniensis . Emerg Infect Dis 13:747–750 [CrossRef]
    [Google Scholar]
  16. Okoli I., Oyeka C. A., Kwon-Chung K. J., Bart T., Robert V., Groenewald J. Z., McFadden D. C., Casadevall A., Boekhout T. 2007; Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar. FEMS Yeast Res 7:339–350 [CrossRef]
    [Google Scholar]
  17. Pagnocca F. C., Carreiro S. C., Bueno O. C., Hebling M. J. A., Silva O. A. 1996; Microbiological changes in leaf-cutting ant nests fed on sesame leaves. J Appl Entomol 120:317–320 [CrossRef]
    [Google Scholar]
  18. Pagnocca F. C., Rodrigues A., Nagamoto N. S., Bacci M. Jr 2008; Yeasts and filamentous fungi carried by the gynes of leaf-cutting ants. Antonie van Leeuwenhoek 94:517–526 [CrossRef]
    [Google Scholar]
  19. Rodrigues A., Bacci M. Jr, Mueller U. G., Ortiz A., Pagnocca F. C. 2008; Microfungal “weeds” in the leafcutter ant symbiosis. Microb Ecol 56:604–614 [CrossRef]
    [Google Scholar]
  20. Sampaio J. P., Gadanho M., Santos S., Duarte F. L., Pais C., Fonseca A., Fell J. W. 2001; Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium : Rhodosporidium kratochvilovae and related anamorphic species. Int J Syst Evol Microbiol 51:687–697
    [Google Scholar]
  21. Sampaio J. P., Inácio J., Fonseca A., Gadanho M., Spencer-Martins I., Scorzetti G., Fell J. 2004; Auriculibuller fuscus gen. nov., sp. nov. and Bullera japonica sp. nov., novel taxa in the Tremellales. Int J Syst Evol Microbiol 54:987–993 [CrossRef]
    [Google Scholar]
  22. Schultz T. R., Brady S. G. 2008; Major evolutionary transitions in ant agriculture. Proc Natl Acad Sci U S A 105:5435–5440 [CrossRef]
    [Google Scholar]
  23. Schultz T. R., Meier R. 1995; A phylogenetic analysis of the fungus-growing ants (Hymenoptera: Formicidae: Attini) based on morphological characters of the larvae. Syst Entomol 20:337–370 [CrossRef]
    [Google Scholar]
  24. Silva A., Bacci M. Jr, Pagnocca F. C., Bueno O. C., Hebling M. J. A. 2006; Starch metabolism in Leucoagaricus gongylophorus , the symbiotic fungus of leaf-cutting ants. Microbiol Res 161:299–303 [CrossRef]
    [Google Scholar]
  25. Sugita T., Takashima M., Nakase T., Ichikawa T., Shinoda T., Nishikawa A. 2002; A basidiomycetous anamorphic yeast, Trichosporon terricola sp. nov. isolated from soil. J Gen Appl Microbiol 48:293–297 [CrossRef]
    [Google Scholar]
  26. Sullivan D. J., Haynes K., Bille J., Boerlin P., Rodero L., Lloyd S., Henman M., Coleman D. 1997; Widespread distribution of oral Candida dubliniensis strains in human immunodeficiency virus-infected individuals. J Clin Microbiol 35:960–964
    [Google Scholar]
  27. Swofford D. L. 2002 paup*: Phylogenetic analysis using parsimony (and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  28. Taj-Aldeen S. J., Al-Ansari N., El Shafei S., Meis J. F., Curfs-Breuker I., Theelen B., Boekhout T. 2009; Molecular identification and susceptibility of Trichosporon species isolated from clinical specimens in Qatar: isolation of Trichosporon dohaense Taj-Aldeen, Meis & Boekhout sp. nov. J Clin Microbiol 47:1791–1799 [CrossRef]
    [Google Scholar]
  29. Weber N. A. 1972 Gardening Ants: the Attines Philadelphia: American Philosophical Society;
    [Google Scholar]
  30. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp 77–100 Edited by Kurtzman C. P, Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015727-0
Loading
/content/journal/ijsem/10.1099/ijs.0.015727-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error