1887

Abstract

A Gram-positive bacterium, designated strain CBMB205, was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. Cells were strictly aerobic, motile, rod-shaped and formed endospores. The best growth was achieved at 30 °C and pH 7.0 in ammonium mineral salts (AMS) medium containing 600 mM methanol. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed strain CBMB205 in a clade with the species , and and revealed pairwise similarities ranging from 98.2 to 99.2 %. DNA–DNA hybridization experiments revealed a low level (<36 %) of DNA–DNA relatedness between strain CBMB205 and its closest relatives. The major components of the fatty acid profile were C anteiso, C iso, C iso and C anteiso. The diagnostic diamino acid of the cell wall was -diaminopimelic acid. The G+C content of the genomic DNA was 45.0 mol%. The lipids present in strain CBMB205 were diphosphatidylglycerol, phosphatidylglycerol, a minor amount of phosphatidylcholine and two unknown phospholipids. The predominant respiratory quinone was MK-7. Studies of DNA–DNA relatedness, morphological, physiological and chemotaxonomic analyses and phylogenetic data based on 16S rRNA gene sequencing enabled strain CBMB205 to be described as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is CBMB205 (=KACC 13105=NCCB 100236).

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2010-10-01
2020-01-21
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Scanning electron micrographs of cells of strain CBMB205 grown on AMS medium after 48 h at 28 °C. Bar, 200 nm.



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