1887

Abstract

Two Gram-negative, motile, rod-shaped bacteria (strains 5317J-18 and 5414S-25) were isolated from air samples collected in the Jeju Island and Suwon region of Korea, respectively. Phylogenetically, strain 5317J-18 was grouped with the genus with byr23-80 as the closest relative (98.8 % sequence similarity). Strain 5414S-25 was affiliated with the genus with CCUG 38318 as the closest relative (98.8 % sequence similarity). The mean DNA–DNA relatedness values between strain 5317J-18 and DSM 18925 and DSM 18055 were 43 and 36 %, respectively. The mean DNA–DNA hybridization values between strain 5414S-25 and KACC 13771, DSM 18925, DSM 16850, KACC 13770, DSM 18055, DSM 17473 and DSM 17472 ranged from 33 to 42 %. Both novel strains had ubiquinone Q-8 as the predominant isoprenoid quinone and summed feature 3 (comprising iso-C 2-OH and/or C 7) and C as the major fatty acids. Both strains also showed similar polar lipid profiles with phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. The DNA G+C contents of strains 5317J-18 and 5414S-25 were 66.1 and 67.8 %, respectively. On the basis of their phenotypic, chemotaxonomic and genotypic characteristics, the new strains represent novel species in the genera and . Strain 5317J-18 (=KACC 12634=DSM 21309) is proposed as the type strain of sp. nov. and strain 5414S-25 (=KACC 12635=DSM 21311) is proposed as the type strain of sp. nov.

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2010-08-01
2019-10-20
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vol. , part 8, pp. 1938 - 1943

Maximum-likelihood phylogenetic tree constructed using 16S rRNA gene sequences, showing the position of strains 5317J-18 and 5414S-25 . Maximum-parsimony phylogenetic tree constructed using 16S rRNA gene sequences, showing the position of strains 5317J-18 and 5414S-25 . Polar lipid profiles of DSM 18925 (a), KACC 12188 (b), strain 5317J-18 (c) and strain 5414S-25 (d) after separation by two-dimensional TLC.

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