1887

Abstract

A novel strain of the genus , KFC91, was isolated from the Kafni glacier of the Himalayas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KFC91 clustered with LMG 22078 (98.9 %) and LMG 6935 (98.7 %). The cell wall peptidoglycan contained meso-diaminopimelic acid as the diamino acid, anteiso-C as the predominant fatty acid and MK-7 as the major isoprenoid quinone. Based on the phylogenetic analysis, chemotaxonomic characteristics and other phenotypic traits, strain KFC91 was assigned to the genus . DNA–DNA hybridization experiments of KFC91 with and showed reassociation values of 39 and 52 %, respectively. Thus, it is proposed that strain KFC91 should be assigned the status of a novel species of the genus and the name proposed is sp. nov., with KFC91 (=NCCB 100252 =DSM 22343) as the type strain.

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2010-08-01
2019-12-06
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Southern blot analysis of genomic DNA of strain KFC91 digested using HI (B), I (P) and both HI and I (B+P) and hybridized with the P-labelled partial 16S rRNA gene of KFC91 amplified using the universal primers 16S1 and 16S2. Relative sizes of bands (kb) are indicated to the right.

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