sp. nov., isolated from a wetland Free

Abstract

A bacterial strain, designated Nsw-4, was isolated from a water sample of Niao-Song Wetland Park in Taiwan and was characterized by using a polyphasic taxonomic approach. Strain Nsw-4 was Gram-negative, aerobic, ivory-coloured, rod-shaped and motile by means of a polar flagellum. Growth occurred at 15–37 °C, pH 6.0–8.0 and 0–2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus and that its closest neighbour was WB 3.4-79 (96.9 %). The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from WB 3.4-79. The major fatty acids were C16 : 17 and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. On the basis of 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain Nsw-4 should be classified as representing a novel species and the second member of the genus , for which the name sp. nov. is proposed. The type strain is Nsw-4 (=BCRC 17934=LMG 24817).

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2010-06-01
2024-03-28
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References

  1. Chen W. M., Laevens S., Lee T. M., Coenye T., de Vos P., Mergeay M., Vandamme P. 2001; Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. Int J Syst Evol Microbiol 51:1729–1735 [CrossRef]
    [Google Scholar]
  2. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  3. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archeon Thermococccus profundus DT5432. Appl Environ Microbiol 61:1502–1506
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;
    [Google Scholar]
  10. Kluge A. G., Farris J. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  11. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  12. Maidak B. L., Cole J. R., Lilburn T. G., Parker C. T. Jr, Saxman P. R., Farris R. J., Garrity G. M., Olsen G. J., Schmidt T. M., Tiedje J. M. 2001; The rdp-II (Ribosomal Database Project. Nucleic Acids Res 29:173–174 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for constructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  17. Stackebrandt E., Lang E., Cousin S., Päuker O., Brambilla E., Kroppenstedt R., Lünsdorf H. 2007; Deefgea rivuli gen. nov., sp. nov., a member of the class Betaproteobacteria . Int J Syst Evol Microbiol 57:639–645 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  20. Wen C. M., Tseng C. S., Cheng C. Y., Li Y. K. 2002; Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. Biotechnol Appl Biochem 35:213–219 [CrossRef]
    [Google Scholar]
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