1887

Abstract

The C3H/HeJBir mouse model of intestinal inflammation was used for isolation of a Gram-positive, rod-shaped, non-spore-forming bacterium (B7) from caecal suspensions. On the basis of partial 16S rRNA gene sequence analysis, strain B7 was a member of the class , family , and was related closely to Mt1B8 (97.6 %). The major fatty acid of strain B7 was C (19.1 %) and the respiratory quinones were mono- and dimethylated. Cells were aerotolerant, but grew only under anoxic conditions. Strain B7 did not convert the isoflavone daidzein and was resistant to cefotaxime. The results of DNA–DNA hybridization experiments and additional physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain B7 from the type strain of . Therefore, strain B7 represents a novel species, for which the name sp. nov. is proposed. The type strain is B7 (=DSM 21839 =CCUG 56815).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.015016-0
2010-07-01
2019-12-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/7/1527.html?itemId=/content/journal/ijsem/10.1099/ijs.0.015016-0&mimeType=html&fmt=ahah

References

  1. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  2. Clavel, T., Charrier, C., Braune, A., Wenning, M., Blaut, M. & Haller, D. ( 2009; ). Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov. Int J Syst Evol Microbiol 59, 1805–1812.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Duck, L. W., Walter, M. R., Novak, J., Kelly, D., Tomasi, M., Cong, Y. & Elson, C. O. ( 2007; ). Isolation of flagellated bacteria implicated in Crohn's disease. Inflamm Bowel Dis 13, 1191–1201.[CrossRef]
    [Google Scholar]
  5. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Kirschner, C., Maquelin, K., Pina, P., Ngo Thi, N. A., Choo-Smith, L. P., Sockalingum, G. D., Sandt, C., Ami, D., Orsini, F. & other authors ( 2001; ). Classification and identification of enterococci: a comparative phenotypic, genotypic, and vibrational spectroscopic study. J Clin Microbiol 39, 1763–1770.[CrossRef]
    [Google Scholar]
  8. Ley, R. E., Backhed, F., Turnbaugh, P., Lozupone, C. A., Knight, R. D. & Gordon, J. I. ( 2005; ). Obesity alters gut microbial ecology. Proc Natl Acad Sci U S A 102, 11070–11075.[CrossRef]
    [Google Scholar]
  9. Ley, R. E., Turnbaugh, P. J., Klein, S. & Gordon, J. I. ( 2006; ). Microbial ecology: human gut microbes associated with obesity. Nature 444, 1022–1023.[CrossRef]
    [Google Scholar]
  10. Maruo, T., Sakamoto, M., Ito, C., Toda, T. & Benno, Y. ( 2008; ). Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella. Int J Syst Evol Microbiol 58, 1221–1227.[CrossRef]
    [Google Scholar]
  11. Matthies, A., Clavel, T., Gutschow, M., Engst, W., Haller, D., Blaut, M. & Braune, A. ( 2008; ). Conversion of daidzein and genistein by an anaerobic bacterium newly isolated from the mouse intestine. Appl Environ Microbiol 74, 4847–4852.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minamida, K., Ota, K., Nishimukai, M., Tanaka, M., Abe, A., Sone, T., Tomita, F., Hara, H. & Asano, K. ( 2008; ). Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum. Int J Syst Evol Microbiol 58, 1238–1240.[CrossRef]
    [Google Scholar]
  14. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S. ( 1955; ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef]
    [Google Scholar]
  15. Rossello-Mora, R. & Amann, R. ( 2001; ). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef]
    [Google Scholar]
  16. Santos, S. R. & Ochman, H. ( 2004; ). Identification and phylogenetic sorting of bacterial lineages with universally conserved genes and proteins. Environ Microbiol 6, 754–759.[CrossRef]
    [Google Scholar]
  17. Sasser,M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  20. Sundberg, J. P., Elson, C. O., Bedigian, H. & Birkenmeier, E. H. ( 1994; ). Spontaneous, heritable colitis in a new substrain of C3H/HeJ mice. Gastroenterology 107, 1726–1735.
    [Google Scholar]
  21. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  22. Visuvanathan, S., Moss, M. T., Standord, J. L., Hermon-Taylor, J. & McFadden, J. J. ( 1989; ). Simple enzymatic method for isolation of DNA from diverse bacteria. J Microbiol Methods 10, 59–64.[CrossRef]
    [Google Scholar]
  23. Wenning, M., Scherer, S. & Naumann, D. ( 2008; ). Infrared spectroscopy in the identification of microorganisms. In Vibrational Spectroscopy for Medical Diagnosis, pp. 71–96. Edited by M. Diem, P. R. Griffith & J. M. Chalmers. Chichester, UK: Wiley.
  24. Whiton, R. S., Lau, P., Morgan, S. L., Gilbart, J. & Fox, A. ( 1985; ). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. J Chromatogr 347, 109–120.[CrossRef]
    [Google Scholar]
  25. Würdemann, D., Tindall, B. J., Pukall, R., Lünsdorf, H., Strömpl, C., Namuth, T., Nahrstedt, H., Wos-Oxley, M., Ott, S., Schreiber, S., Timmis, K. N. & Oxley, A. P. A. ( 2009; ). Gordonibacter pamelaeae gen. nov., sp. nov., a new member of the Coriobacteriaceae isolated from a patient with Crohn's disease and reclassification of Eggerthella hongkongensis Lau et al. 2006 as Paraeggerthella hongkongensis gen. nov., comb. nov. Int J Syst Evol Microbiol 59, 1405–1415.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.015016-0
Loading
/content/journal/ijsem/10.1099/ijs.0.015016-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1527–1531

Comparative polar lipid analysis of (a) strain B7 and (b) DSM 19490 .

Reversed-phase HPLC analysis of daidzein and equol concentrations.

[ Single PDF file] (190 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error