1887

Abstract

A Gram-positive actinobacterium (CC-NMPT-T3) was isolated from iron-ore-contaminated soil near New Mangalore Port, Karnataka, India. Based on 16S rRNA gene sequence similarity studies, strain CC-NMPT-T3 belongs to the genus and is most closely related to (98.6 %), (97.4 %) and (97.4 %). The peptidoglycan is of the type A4 -Lys←-Glu. The predominant isoprenoid quinone is menaquinone MK-8(H) and the polar lipid profile is composed of the predominant compound diphosphatidylglycerol, moderate amounts of a phosphatidylinositol-mannoside, phosphatidylinositol and minor amounts of another phosphatidylmannoside and phosphatidylglycerol. The major fatty acids of strain CC-NMPT-T3 are anteiso-C and iso-C. The results of DNA–DNA hybridizations, and physiological and biochemical tests allowed a clear phenotypic differentiation of strain CC-NMPT-T3 from all other species. Strain CC-NMPT-T3 represents a novel species, for which the name sp. nov. is proposed. The type strain is CC-NMPT-T3 (=DSM 21838=CCM 7658).

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2010-05-01
2019-12-06
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vol. , part 5, pp. 1027-1030

Polar lipid profile of CC-NMPT-T3 separated by 2-dimensional thin layer chromatography and detected with molybdatophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PI, phosphatidylinositol; PIMs, phosphatidylinositol-mannosides; L1, L2, unidentified polar lipids not reacting with the spray reagents specific for a free amino group, phosphate or sugars.



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