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Abstract

A novel halophilic Gram-negative sulfate-reducing bacterium affiliated with the deltaproteobacterial family , strain PropA, was isolated from the extreme hypersaline sediment of the northern arm of Great Salt Lake, Utah, USA. Comparative 16S rRNA gene sequence analysis showed that strain PropA is the first cultured representative of a clade of phylotypes that have been retrieved from a range of geographically and ecologically distinct hypersaline environments. Strain PropA shared ≤90 % 16S rRNA gene sequence identity with cultured strains within the family . Cells of strain PropA were rod-shaped and sometimes motile. The strain required NaCl for growth and grew at salinities up to 200 g NaCl l (optimum 60 g l). Growth was observed at 15–40 °C, optimum growth occurred at about 40 °C, while growth was absent at 10 and 45 °C. The pH range for growth was pH 6.0–8.3. Yeast extract (0.1 g l) was required for growth. C alcohols, C carboxylic acids, yeast extract and H/acetate supported growth with sulfate as electron acceptor. Sulfate, thiosulfate and sulfite served as electron acceptors, but not elemental sulfur, nitrate or fumarate. The DNA G+C content of strain PropA was 54.1 mol%. Based on the genotypic and physiological properties, we propose that strain PropA represents a novel species within a novel genus, gen. nov., sp. nov. The type strain of is PropA (=DSM 17721 =VKM B-2385).

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2010-05-01
2024-03-28
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References

  1. Beliakova E. V., Rozanova E. P., Borzenkov I. A., Tourova T. P., Pusheva M. A., Lysenko A. M., Kolganova T. V. 2006; The new facultatively chemolithoautotrophic, moderately halophilic, sulfate-reducing bacterium Desulfovermiculus halophilus gen.nov., sp. nov., isolated from an oil field. Mikrobiologiia 75:201–211 (in Russian
    [Google Scholar]
  2. Brandt K. K., Ingvorsen K. 1997; Desulfobacter halotolerans sp. nov., a halotolerant acetate-oxidizing sulfate-reducing bacterium isolated from sediments of Great Salt Lake, Utah. Syst Appl Microbiol 20:366–373 [CrossRef]
    [Google Scholar]
  3. Brandt K. K., Patel B. K. C., Ingvorsen K. 1999; Desulfocella halophila gen. nov., sp. nov., a halophilic, fatty-acid-oxidizing, sulfate-reducing bacterium isolated from sediments of the Great Salt Lake. Int J Syst Bacteriol 49:193–200 [CrossRef]
    [Google Scholar]
  4. Brandt K. K., Vester F., Jensen A. N., Ingvorsen K. 2001; Sulfate reduction dynamics and enumeration of sulfate-reducing bacteria in hypersaline sediments of the Great Salt Lake. (Utah, USA: Microb Ecol 41:1–11
    [Google Scholar]
  5. Cravo-Laureau C., Matheron R., Joulian C., Cayol J.-L., Hirschler-Réa A. 2004; Desulfatibacillum alkenivorans sp. nov., a novel n-alkene-degrading, sulfate-reducing bacterium, and emended description of the genus Desulfatibacillum . Int J Syst Evol Microbiol 54:1639–1642 [CrossRef]
    [Google Scholar]
  6. Daffonchio D., Borin S., Brusa T., Brusetti L., van der Wielen P. W., Bolhuis H., Yakimov M. M., D'Auria G., Giuliano L. other authors 2006; Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline. Nature 440:203–207 [CrossRef]
    [Google Scholar]
  7. Drzyzga O., Kuever J., Blotevogel K. H. 1993; Complete oxidation of benzoate and 4-hydroxybenzoate by a new sulfate-reducing bacterium resembling Desulfoarculus . Arch Microbiol 159:109–113 [CrossRef]
    [Google Scholar]
  8. Finster K., Liesack W., Tindall B. J. 1997; Desulfospira joergensenii , gen. nov., sp. nov., a new sulfate-reducing bacterium isolated from marine surface sediment. Syst Appl Microbiol 20:201–208 [CrossRef]
    [Google Scholar]
  9. Foti M., Sorokin D. Y., Lomans B., Mußmann M., Zacharova E. E., Pimenov N. V., Kuenen J. G., Muyzer G. 2007; Diversity, activity, and abundance of sulfate-reducing bacteria in saline and hypersaline soda lakes. Appl Environ Microbiol 73:2093–2100 [CrossRef]
    [Google Scholar]
  10. Glastrup J., Shashoua Y., Egsgaard H., Mortensen M. N. 2006; Formic and acetic acids in archaeological wood. A comparison between the Vasa warship, the Bremen cog, the Oberländer boat and the Danish viking ships. Holzforschung 60:259–264
    [Google Scholar]
  11. Jakobsen T. F., Kjeldsen K. U., Ingvorsen K. 2006; Desulfohalobium utahense sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from Great Salt Lake. Int J Syst Evol Microbiol 56:2063–2069 [CrossRef]
    [Google Scholar]
  12. Kjeldsen K. U., Loy A., Jakobsen T. F., Thomsen T. R., Wagner M., Ingvorsen K. 2007; Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment, Great Salt Lake (Utah. FEMS Microbiol Ecol 60:287–298 [CrossRef]
    [Google Scholar]
  13. Krekeler D., Sigalevich P., Teske A., Cypionka H., Cohen Y. 1997; A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxyclinae sp. nov. Arch Microbiol 167:369–375 [CrossRef]
    [Google Scholar]
  14. Kuever J., Könneke M., Galushko A., Drzyzga O. 2001; Reclassification of Desulfobacterium phenolicum as Desulfobacula phenolica comb.nov. and description of strain SaxT as Desulfotignum balticum gen. nov., sp. nov. Int J Syst Evol Microbiol 51:171–177
    [Google Scholar]
  15. Kuever J., Rainey F. A., Widdel F. 2005a; Family III. Desulfohalobiaceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 2, part C pp 948–949 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  16. Kuever J., Rainey F. A., Widdel F. 2005b; Family IV. Desulfonatronaceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 2, part Cp–956 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  17. Kuever J., Rainey F. A., Widdel F. 2005c; Family I. Desulfobacteraceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol. 2, part C pp 959–960 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  18. Larsen H. 1986; Halophilic and halotolerant microorganisms: an overview and historical perspective. FEMS Microbiol Lett 39:3–7 [CrossRef]
    [Google Scholar]
  19. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  20. Ollivier B., Hatchikian C. E., Prensier G., Guezennec J., Garcia J.-L. 1991; Desulfohalobium retbaense gen. nov., sp. nov., a halophilic sulfate-reducing bacterium from sediments of a hypersaline lake in Senegal. Int J Syst Bacteriol 41:74–81 [CrossRef]
    [Google Scholar]
  21. Oren A. 1999; Bioenergetic aspects of halophilism. Microbiol Mol Biol Rev 63:334–348
    [Google Scholar]
  22. Oren A. 2002 Halophilic Microorganisms and their Environments Dordrecht: Kluwer;
    [Google Scholar]
  23. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35:7188–7196 [CrossRef]
    [Google Scholar]
  24. Rabus R., Nordhaus R., Ludwig W., Widdel F. 1993; Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. Appl Environ Microbiol 59:1444–1451
    [Google Scholar]
  25. Ronquist F., Huelsenbeck J. P. 2003; MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574 [CrossRef]
    [Google Scholar]
  26. Sass H., Cypionka H. 2004; Isolation of sulfate-reducing bacteria from the terrestrial deep subsurface and description of Desulfovibrio cavernae sp. nov. Syst Appl Microbiol 27:541–548 [CrossRef]
    [Google Scholar]
  27. Swofford D. L. 2003 paup*: Phylogenetic analysis using parsimony (and other methods), version 4.0b10 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  28. Wagner M., Loy A., Klein M., Lee N., Ramsing N. B., Stahl D. A., Friedrich M. W. 2005; Functional marker genes for identification of sulfate-reducing prokaryotes. Methods Enzymol 397:469–489
    [Google Scholar]
  29. Widdel F., Bak F. 1992; Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes , 2nd edn. pp 3352–3378 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
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