1887

Abstract

Two strains of anaerobic, Gram-stain-negative bacilli isolated from the human oral cavity (D033B-12-2 and D080A-01) were subjected to a comprehensive range of phenotypic and genotypic tests and were found to be distinct from any previously described species. 16S rRNA gene sequence analysis revealed that the strains were related most closely to the type strain of (93.5 % sequence identity). The novel strains were saccharolytic and produced acetic acid and succinic acid as end products of fermentation. The principal cellular long-chain fatty acids were C, iso-C, C, anteiso-C, iso-C and C 3-OH. The G+C content of the DNA of strain D033B-12-2 was 44 mol%. Strains D033B-12-2 and D080A-01 are considered to represent a single novel species of the genus , for which the name sp. nov. is proposed. The type strain is D033B-12-2 (=DSM 22473 =CCUG 57944).

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2010-10-01
2019-10-20
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vol. , part 10, pp. 2458 - 2461

Phenotypic characteristics of strains of sp. nov.

+, Positive; –, negative; W, weakly positive.

Profile of fatty acid methyl esters of strain D033B-12-2

Phylogenetic trees based on 16S rRNA gene sequence comparisons over 1360 aligned bases showing the relationship between strains D033B-12-2 and D080A-01 and related species based on the neighbour-joining (Fig. S1) and maximum-parsimony (Fig. S2) methods. [ PDF] (49 KB)



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