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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 60, Issue 6

gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters Free

Abstract

A strictly anaerobic, thermophilic bacterium, designated strain AeB, was isolated from microbial mats colonizing a run-off channel formed by free-flowing thermal water from a bore well (registered number 17263) of the Great Artesian Basin, Australia. Cells of strain AeB were slightly curved rods (2.5–6.0×1.0 μm) that stained Gram-negative and formed spherical terminal to subterminal spores. The strain grew optimally in tryptone–yeast extract–Casamino acids medium at 50 °C (range 37–55 °C) and pH 7 (range pH 5–9). Strain AeB grew poorly on yeast extract (0.2 %) and tryptone (0.2 %) as sole carbon sources, which were obligately required for growth on other energy sources. Growth of strain AeB increased in the presence of various carbohydrates and amino acids, but not organic acids. End products detected from glucose fermentation were ethanol, acetate, CO and H. In the presence of 0.2 % yeast extract, iron(III), manganese(IV), vanadium(V) and cobalt(III) were reduced, but not sulfate, thiosulfate, sulfite, elemental sulfur, nitrate or nitrite. Iron(III) was also reduced in the presence of tryptone, peptone, Casamino acids and amyl media (Research Achievement), but not starch, xylan, chitin, glycerol, ethanol, pyruvate, benzoate, lactate, acetate, propionate, succinate, glycine, serine, lysine, threonine, arginine, glutamate, valine, leucine, histidine, alanine, aspartate, isoleucine or methionine. Growth was inhibited by chloramphenicol, streptomycin, tetracycline, penicillin, ampicillin and NaCl concentrations >2 %. The DNA G+C content was 35.4±1 mol%, as determined by the thermal denaturation method. 16S rRNA gene sequence analysis indicated that strain AeB is a member of the family , class phylum ‘’, and is positioned approximately equidistantly between the genera , , and (16S rRNA gene similarity values of 87.8–90.9 %). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain AeB is considered to represent a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain is AeB (=JCM 15555=KCTC 5667).

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Keyword(s): GAB, Great Artesian Basin
SGM
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2010-06-01
2025-01-26
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Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters
Int J Syst Evol Microbiol 60, 1394 (2010); https://doi.org/10.1099/ijs.0.014670-0
/content/journal/ijsem/10.1099/ijs.0.014670-0
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