1887

Abstract

A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain DCY01, was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY01 belonged to the and was most closely related to KCTC 12211 (98.4 % similarity), R-32729 (97.2 %), R-32768 (97.1 %), DSM 50170 (96.9 %) and DSM 12575 (96.8 %). Chemotaxonomic analyses revealed that strain DCY01 possessed a quinone system with Q-8 as the predominant compound, and iso-C (28.2 %), C 10methyl (13.2 %), iso-C F (10.8 %) and C (7.5 %) as major fatty acids, corroborating assignment of strain DCY01 to the genus . The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The results of DNA–DNA hybridization and physiological and biochemical tests clearly demonstrated that strain DCY01 represents a species distinct from recognized species. Based on these data, DCY01 (=KCTC 12539=NBRC 101154) should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2010-07-01
2019-10-14
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vol. , part 7, pp. 1522-1526

Two-dimensional thin-layer chromatogram of polar lipids of strain DCY01 . Cellular fatty acid profiles of strain DCY01 and the type strains of related species. Levels of DNA–DNA relatedness between strain DCY01 and the type strains of related species. [Combined PDF](94 KB)



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