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Abstract

Seven strains of purple nonsulfur bacteria isolated from the shallow-water steppe soda lakes of the cryoarid zone of Central Asia formed a genetically homogeneous group within the genus . The isolates were most closely related to from which they differed at the species level (99.5 % 16S rRNA gene identity and 42–44 % DNA–DNA hybridization level). According to genotypic and phenotypic characteristics, the strains were assigned to a new species of the genus , for which the name sp. nov. is proposed. Cells of all strains were ovoid to rod-shaped, 0.3–0.8 μm wide and 1–2.5 μm long, and motile by means of polar flagella. They contained internal photosynthetic membranes of the vesicular type and photosynthetic pigments (bacteriochlorophyll and carotenoids of the spheroidene series). All strains were obligate haloalkaliphiles, growing within a wide range of salinity (0.3–10 %) and pH (7.5–10), with growth optima at 1–5 % NaCl and pH 8.5. Photo- and chemoheterotrophic growth occurred with a number of organic compounds and biotin, thiamine and niacin as growth factors. No anaerobic respiration on nitrite, nitrate or fumarate and no fermentation was demonstrated. Bacteria grew photo- and chemolithoautotrophically with sulfide, sulfur and thiosulfate, oxidizing them to sulfate. Sulfide was oxidized via deposition of extracellular elemental sulfur. No growth with H as electron donor was demonstrated. The major fatty acid was 18 : 1 (81.0 %). The major quinone was Q-10. The DNA G+C content was 66.1 mol% ( ). The type strain, A-20s (=VKM B-2489 =DSM 21153), was isolated from soda lake Khilganta (Zabaikal'skii Krai, southern Siberia, Russia).

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2010-05-01
2024-12-05
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References

  1. Boldareva E. N., Akimov V. N., Boychenko V. A., Stadnichuk I. N., Moskalenko A. A., Makhneva Z. K., Gorlenko V. M. 2008; Rhodobaca barguzinensis sp. nov., a new alkaliphilic purple nonsulfur bacterium isolated from a soda lake of the Barguzin Valley (Buryat Republic, Eastern Siberia). Microbiology (English translation of Mikrobiologiia ) 77:206–218
    [Google Scholar]
  2. Hiraishi A., Ueda Y. 1995; Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int J Syst Bacteriol 45:319–326 [CrossRef]
    [Google Scholar]
  3. Imhoff J. F. 2001; The anoxygenic phototrophic purple bacteria. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 621–627 Edited by Brenner D. Y., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  4. Imhoff J. F. 2008; Systematics of anoxygenic phototrophic bacteria. In Sulfur Metabolism in Phototrophic Organisms pp 269–287 Edited by Hell R., Dahl C., Knaff D., Leustek Th. New York: Springer;
    [Google Scholar]
  5. Kompantseva E. I., Sorokin D. Yu., Gorlenko V. M., Namsaraev B. B. 2005; The phototrophic community found in lake Khilganta (an alkaline saline lake located in the Southeastern Transbaikal region). Microbiology (English translation of Mikrobiologiia ) 74:352–361
    [Google Scholar]
  6. Kompantseva E. I., Bryantseva I. A., Komova A. V., Namsaraev B. B. 2007; The structure of phototrophic communities of soda lakes of the southeastern Transbaikal region. Microbiology (English translation of Mikrobiologiia ) 76:211–219
    [Google Scholar]
  7. Kompantseva E. I., Komova A. V., Krauzova V. I., Kolganova T. V., Panteleeva A. V. 2009; Purple nonsulfur bacteria in weakly and moderately mineralized soda lakes of the southern Transbaikal region and northeastern Mongolia. Microbiology (English translation of Mikrobiologiia ) 78:246–253
    [Google Scholar]
  8. Milford A. D., Achenbach L. A., Jung D. O., Madigan M. T. 2000; Rhodobaca bogoriensis gen. nov. and sp. nov., an alkaliphilic purple nonsulfur bacterium from African Rift Valley soda lakes. Arch Microbiol 174:18–27 [CrossRef]
    [Google Scholar]
  9. Owen R. J., Lapage S. P. 1976; The thermal denaturation of partly purified bacterial deoxyribonucleic acid and its taxonomic applications. J Appl Bacteriol 41:335–340 [CrossRef]
    [Google Scholar]
  10. Pfennig N., Lippert K. D. 1966; Über das Vitamin B12-Bedürfnis phototropher Schwefelbacterien. Arch Microbiol 55:245–256
    [Google Scholar]
  11. Ryter A., Kellenberger E. 1958; Etude au microscope électronique des plasmas contenant de l'acide désoxyribonucléique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch [B] 13b:597–605
    [Google Scholar]
  12. Stead D. E., Sellwood J. E., Wilson J., Viney I. 1992; Evaluation of a commercial microbial identification system based on fatty acid profiles for rapid, accurate identification of plant pathogenic bacteria. J Appl Bacteriol 72:315–321 [CrossRef]
    [Google Scholar]
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