Monoxenous trypanosomatid Herpetomonas trimorpha sp. nov. was isolated from the digestive tract of the biting midge Culicoides truncorum (Ceratopogonidae, Diptera). This species forms three distinct morphotypes in culture: the microflagellate promastigote, the small promastigote and the long promastigote. The last form is unique for the newly described species. Phylogenetic analyses of SSU rRNA and glycosomal glyceraldehyde phosphate dehydrogenase genes showed that H. trimorpha sp. nov. is the closest relative of Herpetomonas ztiplika, another monoxenous trypanosomatid isolated from biting midges. However, morphological and randomly amplified polymorphic DNA analyses confirmed that H. trimorpha sp. nov. is distinct from H. ztiplika.
BulatS. A.,
MokrousovI. V.,
PodlipaevS. A.1999; Classification of Trypanosomatids from insects and plants by the UP-PCR (universally primed PCR) technique and cross dot blot hybridization of PCR products. Eur J Protistol 35:319–326[CrossRef]
CamargoE. P.,
SbravateC.,
TeixeiraM. M. G.,
UlianaS. R. B.,
SoaresM. B. M.,
AffonsoH. T.,
Floeter-WinterL.1992; Ribosomal DNA restriction analysis and synthetic oligonucleotide probing in the identification of genera of lower trypanosomatids. J Parasitol 78:40–48[CrossRef]
CatarinoL. M.,
SerranoM. G.,
CavazzanaM.Jr,
AlmeidaM. L.,
KaneshinaE. K.,
CampanerM.,
JankeviciusJ. V.,
TeixeiraM. M. G.,
Itow-JankeviciusS.2001; Classification of trypanosomatids from fruits and seeds using morphological, biochemical and molecular markers revealed several genera among fruit isolates. FEMS Microbiol Lett 201:65–72[CrossRef]
Faria-e-SilvaP. M.,
SoaresM. J.,
De SouzaW.1996; Proliferative opisthomastigote forms in Herpetomonas roitmani (Kinetoplastida: Trypanosomatidae). Parasitol Res 82:125–129[CrossRef]
FioriniJ. E.,
TakataC. S. A.,
TeofiloV. M.,
NascimentoL. C.,
Faria-e-SilvaP. M.,
SoaresM. J.,
TeixeiraM. M. G.,
De SouzaW.2001; Morphological, biochemical and molecular characterization of Herpetomonas samuelpessoai camargoi n. subsp., a trypanosomatid isolated from the flower of the squash Cucurbita moschata
. J Eukaryot Microbiol 48:62–69[CrossRef]
FreymullerE.,
CamargoE. P.1981; Ultrastructural differences between species of trypanosomatids with and without endosymbionts. J Protozool 28:175–182[CrossRef]
FrolovA. O.,
SkarlatoS. O.1987; Light- and electron microscopical study of Leptomonas pyrrhocoris Z. (Kinetoplastida, Trypanosomatidae). Parazitologiia 21:3–9 (in Russian with English summary
HallT. A.1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
HamiltonP. B.,
StevensJ. R.,
GauntM. W.,
GidleyJ.,
GibsonW. C.2004; Trypanosomes are monophyletic: evidence from genes for glyceraldehyde phosphate dehydrogenase and small subunit ribosomal RNA. Int J Parasitol 34:1393–1404[CrossRef]
HughesA. L.,
PiontkivskaH.2003; Phylogeny of Trypanosomatidae and Bodonidae (Kinetoplastida) based on 18S rRNA: evidence for paraphyly of Trypanosoma and six other genera. Mol Biol Evol 20:644–652[CrossRef]
ManaiaA. C.,
SouzaM. C. M.,
LustosaÉ. S.,
RoitmanI.1981; Leptomonas lactosovorans n. sp., a lactose-utilizing trypanosomatid: description and nutritional requirements. J Eukaryot Microbiol 28:124–126
MaslovD. A.,
LukešJ.,
JirkůM.,
SimpsonL.1996; Phylogeny of trypanosomes as inferred from the small and large subunit rRNAs: implications for the evolution of parasitism in the trypanosomatid protozoa. Mol Biochem Parasitol 75:197–205[CrossRef]
MaslovD. A.,
PodlipaevS. A.,
LukešJ.2001; Phylogeny of the Kinetoplastida: taxonomic problems and insights into the evolution of parasitism. Mem Inst Oswaldo Cruz 96:397–402[CrossRef]
MerzlyakE.,
YurchenkoV.,
KolesnikovA. A.,
AlexandrovK.,
PodlipaevS. A.,
MaslovD. A.2001; Diversity and phylogeny of insect trypanosomatids based on small subunit rRNA genes: polyphyly of Leptomonas and Blastocrithidia
. J Eukaryot Microbiol 48:161–169[CrossRef]
MorsyT. A.,
SchnurL. F.,
FeinsodF. M.,
MichaelS. A.,
SaahA.,
SalmaM. M.,
WahbaM. M.1988; The discovery and preliminary characterization of a novel trypanosomatid parasite from Rattus norvegicus and stray dogs from Alexandria. Egypt. Ann Trop Med Parasitol 82:437–444
NunesL. R.,
TeixeiraM. M. G.,
CamargoE. P.,
AffonsoH. T.1994; kDNA and rDNA sequences reveal a phylogenetic cluster of species originally placed in different genera of trypanosomatids. J Eukaryot Microbiol 41:496–500[CrossRef]
PavlíčekA.,
HrdáŠ.,
FlegrJ.1999; FreeTree – freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of the genus Frenkelia
. Folia Biol (Praha 45:97–99
PodlipaevS. A.1985; New species of lower trypanosomatids from Heteroptera families Gerridae and Nabidae: stages of their life cycles in nature and in the laboratory. Proc Zool Inst Leningrad 129:35–47 (in Russian with English summary
PodlipaevS. A.,
SturmN. R.,
FialaI.,
FernandesO.,
WestenbergerS. J.,
DolletM.,
CampbellD. A.,
LukešJ.2004a; Diversity of insect trypanosomatids assessed from the spliced leader RNA and 5S rRNA genes and intergenic regions. J Eukaryot Microbiol 51:283–290[CrossRef]
SantosA. L. S.,
AbreuC. M.,
AlvianoC. S.,
SoaresR. M. A.2005; Use of proteolytic enzymes as an addititional tool for trypanosomatid identification. Parasitology 130:79–88[CrossRef]
TeixeiraM. M. G.,
TakataC. S. A.,
ConchonI.,
CampanerM.,
CamargoE. P.1997; Ribosomal and kDNA markers distinguish two subgroups of Herpetomonas among old species and new trypanosomatids isolated from flies. J Parasitol 83:58–65[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
WallaceF. G.,
CamargoE. P.,
McGheeR. B.,
RoitmanI.1983; Guidelines for the description of new species of lower trypanosomatids. J Eukaryot Microbiol 30:308–313
YurchenkoV.,
LukešJ.,
XuX.,
MaslovD. A.2006 An integrated morphological and molecular approach to a new species description in the Trypanosomatidae: the case of Leptomonas podlipaevi n. sp., a parasite of Boisea rubrolineata (Hemiptera: Rhopalidae) J Eukaryot Microbiol 53:103–111[CrossRef]
YurchenkoV. Y.,
LukešJ.,
TesařováM.,
JirkůM.,
MaslovD. A.2008; Morphological discordance of the new trypanosomatid species phylogenetically associated with the genus Crithidia
. Protist 159:99–114[CrossRef]
YurchenkoV. Y.,
LukešJ.,
JirkůM.,
MaslovD. A.2009; Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera. Int J Syst Evol Microbiol 59:893–909[CrossRef]