sp. nov., an extremely halophilic archaeon from solar salt Free

Abstract

A novel extremely halophilic archaeon, strain 194-10, was isolated from a solar salt sample imported into Japan from the Philippines. Strain 194-10 was pleomorphic, neutrophilic and mesophilic and required at least 10 % (w/v) NaCl but no MgSO . 7HO for growth; it exhibited optimal growth at 15 % (w/v) NaCl and 60 mM MgSO . 7HO. Strain 194-10 grew at 20–45 °C (optimum, 30 °C) and pH 6.0–9.0 (optimum, pH 6.5–7.0). The G+C content of its DNA was 59.8 mol%. 16S rRNA gene sequence analysis revealed closest proximity to XH-48 (98.5 % similarity), the sole representative of the genus . Polar lipid analysis revealed that strain 194-10 contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester (the latter derived from both CC and CC archaeol) and several unidentified glycolipids. The results of DNA–DNA hybridization (20.7 % relatedness between JCM 13463 and strain 194-10) and physiological and biochemical characteristics allowed differentiation of strain 194-10 from XH-48. Therefore, strain 194-10 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain 194-10 (=DSM 22427 =JCM 16110 =CECT 7536).

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2010-12-01
2024-03-29
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