1887

Abstract

The taxonomic positions of two Gram-positive, endospore-forming rods, strains CCUG 53915 and CCUG 53480, isolated from an industrial clean-room floor and from a human blood sample, respectively, were studied. 16S rRNA gene sequence similarity studies revealed that both isolates clearly clustered with species. Strain CCUG 53915 was most closely related to and , showing 99.4 and 99.2 % 16S rRNA gene sequence similarities to the type strains of these species, respectively. Strain CCUG 53480 showed the highest 16S rRNA gene sequence similarities to the type strains of (98.7 %) and (98.6 %). Strains CCUG 53915 and CCUG 53480 had peptidoglycan type A4 -Lys–-Glu. The quinone systems of both strains were composed predominantly of menaquinone MK-7, with small amounts of MK-8. The polar lipid profiles of both strains consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and three unidentified phospholipids. The fatty acid profiles, which comprise anteiso- and iso-branched fatty acids, supported affiliation of the two isolates to the genus . The results of physiological and biochemical tests and DNA–DNA hybridization data allowed a clear phenotypic and genotypic differentiation of both strains from the most closely related species. For this reason, it is proposed that strains CCUG 53915 (=DSM 22204) and CCUG 53480 (=DSM 22203) represent two novel species in the genus , with the names sp. nov. and sp. nov., respectively.

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2010-06-01
2020-01-19
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