1887

Abstract

Two bacterial strains, MC-246 and MC-247, were isolated from municipal urban waste compost and characterized by a polyphasic approach. Both isolates were Gram-stain-variable, endospore-forming rods that were catalase-, oxidase- and -galactosidase-positive, and able to grow at 25–50 °C and pH 7.0–9.0, with optimum growth at 37 °C and pH 7. The predominant cellular fatty acids were anteiso-C, iso-C, iso-C, anteiso-C and iso-C; the major respiratory quinone was menaquinone MK-7; the cell wall peptidoglycan was of type A1; and the DNA G+C content was 49 mol%. These characteristics, as well as data from 16S RNA gene sequence analysis, showed that these strains were affiliated with the genus ; the type strains of and were among their closest neighbours (<94.2 % sequence similarity). Nevertheless, the hypothesis that strains MC-246 and MC-247 could represent a novel species was supported by the low 16S rRNA gene sequence similarity values shared with other members of the genus and by the observation of distinct biochemical and physiological traits. Strains MC-246 and MC-247 shared 99.6 % 16S rRNA gene sequence similarity and showed almost identical MALDI-TOF mass spectra, but could be distinguished at the phenotypic and genotypic level. However, DNA–DNA hybridization between strains MC-246 and MC-247 resulted in values above 70 % indicating that both organisms represent a single species, for which the name sp. nov. is proposed; the type strain is MC-246 (=DSM 22072 =CCUG 57263).

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2010-10-01
2020-01-20
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Phylogenetic tree based on 16S rRNA gene sequences showing the nearest neighbours of strains MC-246 and MC-247. The tree was generated by the neighbour-joining method and bootstrap values were generated from 1000 resamplings; only values greater than 50% are shown. Bar, 1 substitution per 200 nt positions. [ PDF] (93 KB)

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RI RiboPrints of strains MC-246 , MC-247, DSM 22253 and DSM 18677 .

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RAPD profiles of strains MC-246 (1), MC-247 (2), DSM 22253 (3) and DSM 18677 (4), with primers M13 and OPA3.

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. Dendrogram generated by the BioTyper software (version 2.0, Bruker Daltonics) showing the similarity of MALDI-TOF mass spectra of cell extracts of strains MC-246 and MC-247 as well as of selected phylogenetically related type strains of the genus .

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