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Abstract

A whitish Gram-negative, motile, aerobic bacterium, designated strain H 14, was isolated from seawater collected at St Kilda beach in Port Phillip Bay, Melbourne, Australia. Analysis of 16S rRNA gene sequences revealed that the organism belonged to the lineage of the class , forming a distinct evolutionary lineage at the genus level. Strain H 14 was distantly related to the genera , and (family ). Strain H 14 was unable to degrade gelatin, casein, chitin, agar and starch, did not produce any carotenoids, did not possess bacteriochlorophyll and had a limited ability to utilize carbon sources. Strain H 14 grew with concentrations of 1–8 % (w/v) NaCl and over a temperature range of 5–35 °C. Phosphatidylglycerol was the major phospholipid (90 %); phosphatidylcholine (7.9 %) and phosphatidylethanolamine (2.0 %) were present in minor quantities. The predominant fatty acids were C 7 (82.4 %), C 9 (5.1 %) and C (3.8 %). The DNA G+C composition for strain H 14 was 59.1 mol%. Based on the results of physiological, biochemical, chemotaxonomic and phylogenetic investigations, a new genus, gen. nov., with the type species sp. nov. is proposed. The type strain of the type species is H 14 (=KMM 6012=CIP 109922).

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2010-07-01
2019-09-15
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References

  1. Arahal, D. R., Macian, M. C., Garay, E. & Pujalte, M. J. ( 2005; ). Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov. Int J Syst Evol Microbiol 55, 2371–2376.[CrossRef]
    [Google Scholar]
  2. Bligh, E. G. & Dyer, W. J. ( 1959; ). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.[CrossRef]
    [Google Scholar]
  3. Boettcher, K. J., Geaghan, K. K., Maloy, A. P. & Barber, B. J. ( 2005; ). Roseovarius crassostreae sp. nov., a member of the Roseobacter clade and the apparent cause of juvenile oyster disease (JOD) in cultured Eastern oysters. Int J Syst Evol Microbiol 55, 1531–1537.[CrossRef]
    [Google Scholar]
  4. Chevenet, F., Brun, C., Banuls, A. L., Jacq, B. & Christen, R. ( 2006; ). TreeDyn: towards dynamic graphics and annotations for analyses of trees BMC. Bioinformatics 7, 439
    [Google Scholar]
  5. Cho, J.-C. & Giovannoni, S. J. ( 2004; ). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54, 1129–1136.[CrossRef]
    [Google Scholar]
  6. Choi, D. H. & Cho, B. C. ( 2006; ). Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. Int J Syst Evol Microbiol 56, 1869–1873.[CrossRef]
    [Google Scholar]
  7. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  8. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). SeaView and phylo_win, two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  9. Gascuel, O. ( 1997; ). bionj: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 14, 685–695.[CrossRef]
    [Google Scholar]
  10. Gosink, J. J., Herwig, R. P. & Staley, J. T. ( 1997; ). Octadecabacter arcticus gen. nov., sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 20, 356–365.[CrossRef]
    [Google Scholar]
  11. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  12. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  13. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, F. G., Garagulya, A. G., Gorshkova, N. M., Yumoto, N. & Yoshikawa, S. ( 1996; ). Characterization and identification of marine Alteromonas nigrifaciens strains and emendation of the description. Int J Syst Bacteriol 46, 223–228.[CrossRef]
    [Google Scholar]
  14. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, Y. & Yoshikawa, S. ( 1998; ). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef]
    [Google Scholar]
  15. Ivanova, E. P., Gorshkova, N. M., Sawabe, T., Zhukova, N. V., Hayashi, K., Kurilenko, V. V., Alexeeva, Y., Buljan, V., Nicolau, D. V. & other authors ( 2004; ). Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively, from a starfish (Stellaster equestris) and sea grass (Zostera marina). Int J Syst Evol Microbiol 54, 475–480.[CrossRef]
    [Google Scholar]
  16. Ivanova, E. P., Bowman, J. P., Lysenko, A. M., Zhukova, N. V., Gorshkova, N. M., Kuznetsova, T. A., Kalinovskaya, N. L., Shevchenko, L. S. & Mikhailov, V. V. ( 2005; ). Erythrobacter vulgaris sp. nov., a novel organism isolated from the marine invertebrates. Syst Appl Microbiol 28, 123–130.[CrossRef]
    [Google Scholar]
  17. Kim, B.-Y., Weon, H., Hong, S.-B., Jeon, Y.-A., Koo, B.-S. & Kwon, S.-W. ( 2009; ). Thalassobacter arenae sp. nov., isolated from sea sand in Korea. Int J Syst Evol Microbiol 59, 487–490.[CrossRef]
    [Google Scholar]
  18. Kóvacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703
    [Google Scholar]
  19. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. Int J Syst Bacteriol 49, 137–147.[CrossRef]
    [Google Scholar]
  20. Labrenz, M., Lawson, P. A., Tindall, B. J., Collins, M. D. & Hirsch, P. ( 2005; ). Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. Int J Syst Evol Microbiol 55, 41–47.[CrossRef]
    [Google Scholar]
  21. Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., Valentin, F., Wallace, I. M., Wilm, A. & other authors ( 2007; ). clustal w and clustal x version 2.0. Bioinformatics 23, 2947–2948.[CrossRef]
    [Google Scholar]
  22. Macian, M. C., Arahal, D. R., Garay, E., Ludwig, W., Schleifer, K. H. & Pujalte, M. J. ( 2005; ). Jannaschia rubra sp. nov., a red-pigmented bacterium isolated from sea water. Int J Syst Evol Microbiol 55, 649–653.[CrossRef]
    [Google Scholar]
  23. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  24. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  25. Martens, T., Heidorn, T., Pukall, R., Simon, M., Tindall, B.J. & Brinkhoff, T. ( 2006; ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., antibiotic-producing members of the Roseobacter clade, reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1998 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera. Int J Syst Evol Microbiol 56, 1293–1304.[CrossRef]
    [Google Scholar]
  26. Pujalte, M. J., Macian, M. C., Arahal, D. R., Ludwig, W., Schleifer, K. H. & Garay, E. ( 2005; ). Nereida ignava gen. nov., sp. nov., a novel aerobic marine α-proteobacterium that is closely related to uncultured Prionitis (alga) gall symbionts. Int J Syst Evol Microbiol 55, 631–636.[CrossRef]
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  28. Soller, R., Hirsch, P., Blohm, D. & Labrenz, M. ( 2000; ). Differentiation of newly described Antarctic bacterial isolates related to Roseobacter species based on 16S–23S rDNA internal transcribed spacer sequences. Int J Syst Evol Microbiol 50, 909–915.[CrossRef]
    [Google Scholar]
  29. Uchino, Y., Hirata, A., Yokota, A. & Sugiyama, J. ( 1998; ). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44, 201–210.[CrossRef]
    [Google Scholar]
  30. Urbance, J. W., Bratina, B. J., Stoddard, S. F. & Schmidt, T. M. ( 2001; ). Taxonomic characterization of Ketogulonigenium vulgare gen. nov., sp. nov. and Ketogulonigenium robustum sp. nov., which oxidize l-sorbose to 2-keto-l-gulonic acid. Int J Syst Evol Microbiol 51, 1059–1070.[CrossRef]
    [Google Scholar]
  31. Vandecandelaere, I., Nercessian, O., Segaert, E., Achouak, W., Mollica, A., Faimali, M. & Vandamme, P. ( 2009; ). Nautella italica gen. nov., sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 59, 811–817.[CrossRef]
    [Google Scholar]
  32. Vaskovsky, V. E. & Terekhova, T. A. ( 1979; ). HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resolut Chromatogr 2, 671–672.[CrossRef]
    [Google Scholar]
  33. Webb, H. K., Crawford, R. J., Sawabe, T. & Ivanova, E. P. ( 2009; ). Poly(ethylene terephthalate) polymer surfaces as a substrate for bacterial attachment and biofilm formation. Microbes Environ 24, 39–42.[CrossRef]
    [Google Scholar]
  34. Yoon, J. H., Lee, S. Y., Kang, S. J., Lee, C. H. & Oh, T. K. ( 2007; ). Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 542–547.[CrossRef]
    [Google Scholar]
  35. Yoon, J. H., Kang, S. J. & Oh, T. K. ( 2008; ). Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 58, 1198–1202.[CrossRef]
    [Google Scholar]
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vol. , part 7, pp. 1620 - 1625

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vol. , part 7, pp. 1620 - 1625

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Two-dimensional TLC of the polar lipids of strain H 14 . AL, Unidentified aminolipid, PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; LPE, lysophosphatidylethanolamine.

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Phylogenetic position of H 14 according to the 16S rRNA gene sequence analysis (derived from maximum-likelihood analysis). [ Combined PDF] 61 KB.

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Polar lipid and cellular fatty acid composition of H 14 . [ PDF] 41 KB

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