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An aerobic, Gram-negative, yellow-pigmented bacterial strain, designated 0533T, was isolated from frozen soil from the China No. 1 glacier. Phylogenetic analysis of the 16S rRNA gene sequence demonstrated that strain 0533T was a member of the genus Flavobacterium and exhibited 97.1–98.7 % 16S rRNA sequence similarity with its nearest phylogenetic neighbours. Strain 0533T exhibited phenotypic and chemotaxonomic characteristics common to the genus Flavobacterium: menaquinone-6 (MK-6) was the predominant quinone and iso-C15 : 0, C17 : 1 ω6c, anteiso-C15 : 0, iso-C15 : 0 3-OH, C15 : 1 ω6c, iso-C16 : 0 3-OH, summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15 : 1 G and iso-C17 : 0 3-OH were the major fatty acids (>5 %). The DNA G+C content was 32.5 mol%. On the basis of phenotypic and genotypic data, a novel species, Flavobacterium sinopsychrotolerans sp. nov., is proposed. The type strain is 0533T (=CGMCC 1.8704T =JCM 16398T).
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