1887

Abstract

Four isolates (FSL S4-120, FSL S4-696, FSL S4-710, and FSL S4-965) of Gram-positive, motile, facultatively anaerobic, non-spore-forming bacilli that were phenotypically similar to species of the genus were isolated from soil, standing water and flowing water samples obtained from the natural environment in the Finger Lakes National Forest, New York, USA. The four isolates were closely related to one another and were determined to be the same species by whole genome DNA–DNA hybridization studies (>82 % relatedness at 55 °C and >76 % relatedness at 70 °C with 0.0–0.5 % divergence). 16S rRNA gene sequence analysis confirmed their close phylogenetic relatedness to and and more distant relatedness to and Phylogenetic analysis of partial sequences for and showed that these isolates form a well-supported sistergroup to . The four isolates were sufficiently different from and by DNA–DNA hybridization to warrant their designation as a new species of the genus . The four isolates yielded positive reactions in the AccuProbe test that is purported to be specific for , did not ferment -rhamnose, were non-haemolytic on blood agar media, and did not contain a homologue of the virulence gene island. On the basis of their phenotypic characteristics and their genotypic distinctiveness from and the four isolates should be classified as a new species within the genus , for which the name sp. nov. is proposed. The type strain of is FSL S4-120 (=ATCC BAA-1595 =BEIR NR 9579 =CCUG 56148). has not been associated with human or animal disease at this time.

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2010-06-01
2019-10-23
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RI ribotype patterns for the four FSL S4-isolates and the type strains of (ATCC 15313 ) and (ATCC 33090 ).

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Pulsed-field gel electrophoresis patterns for the 4 FSL S4-isolates, 44 isolates used in the ILSINA study, (ATCC 15313 ) and (ATCC 33090 ) type strains, and 1 additional isolate. [ PDF] (628 KB)

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