1887

Abstract

Three Gram-positive, rod-shaped bacteria (strains CC-SBCK-209, CC-12309 and CC-5209) were isolated from the stalk of the edible mushroom grown in the laboratory. 16S rRNA gene sequence analysis indicated that all three isolates clearly belonged to the genus . Strains CC-SBCK-209 and CC-12309 were most related closely to the type strain of (95.9 and 96.1 % 16S rRNA gene sequence similarity, respectively). These two novel strains shared 97.9 % 16S rRNA gene sequence similarity. Levels of similarity to the type strains of all other recognized species were lower than 95.5 %. The third strain (CC-5209) showed the highest 16S rRNA gene sequence similarity to the type strain of (97.6 %); levels of similarity to the type strains of all other recognized species were lower than 96 %. The quinone systems of strains CC-SBCK-209, CC-12309 and CC-5209 consisted of MK-11/MK-12, MK-11/MK-10 and MK-13 as major compounds, respectively. All three strains contained ornithine in their peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The polyamine pattern consisted of spermidine and spermine as predominant components. Fatty acid profiles (anteiso-C, iso-C and anteiso-C as major components) supported the affiliation of all three strains to the genus . The results of physiological and biochemical tests and DNA–DNA hybridization experiments allowed the clear phenotypic and genotypic differentiation of strains CC-SBCK-209 and CC-12309 from and other closely related species. Strain CC-5209 could be differentiated clearly from both genotypically and phenotypically. Based on these data, the novel strains are considered to represent three novel species of the genus . The names proposed for these organisms are sp. nov. [type strain CC-SBCK-209 (=DSM 21798=CCM 7686)], sp. nov. [type strain CC-12309 (=DSM 21799=CCM 7687)] and sp. nov. [type strain CC-5209 (=DSM 22185=CCM 7688)].

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2010-04-01
2019-12-15
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vol. , part 4, pp. 854 - 860

Fatty acid profiles of strains CC-12309 , CC-SBCK-209 and CC-5209 and the type strains of the most closely related species. [ PDF] 52 KB



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