1887

Abstract

Three Gram-positive, rod-shaped bacteria (strains CC-SBCK-209, CC-12309 and CC-5209) were isolated from the stalk of the edible mushroom grown in the laboratory. 16S rRNA gene sequence analysis indicated that all three isolates clearly belonged to the genus . Strains CC-SBCK-209 and CC-12309 were most related closely to the type strain of (95.9 and 96.1 %16S rRNA gene sequence similarity, respectively). These two novel strains shared 97.9 % 16S rRNA gene sequence similarity. Levels of similarity to the type strains of all other recognized species were lower than 95.5 %. The third strain (CC-5209) showed the highest 16S rRNA gene sequence similarity to the type strain of (97.6 %); levels of similarity to the type strains of all other recognized species were lower than 96 %. The quinone systems of strains CC-SBCK-209, CC-12309 and CC-5209 consisted of MK-11/MK-12, MK-11/MK-10 and MK-13 as major compounds, respectively. All three strains contained ornithine in their peptidoglycan. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The polyamine pattern consisted of spermidine and spermine as predominant components. Fatty acid profiles (anteiso-C, iso-C and anteiso-C as major components) supported the affiliation of all three strains to the genus . The results of physiological and biochemical tests and DNA–DNA hybridization experiments allowed the clear phenotypic and genotypic differentiation of strains CC-SBCK-209 and CC-12309 from and other closely related species. Strain CC-5209 could be differentiated clearly from both genotypically and phenotypically. Based on these data, the novel strains are considered to represent three novel species of the genus . The names proposed for these organisms are sp. nov. [type strain CC-SBCK-209 (=DSM 21798=CCM 7686)], sp. nov. [type strain CC-12309 (=DSM 21799=CCM 7687)] and sp. nov. [type strain CC-5209 (=DSM 22185=CCM 7688)].

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014092-0
2010-04-01
2020-09-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/4/854.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014092-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Akimov V. N., Lubitz W., Busse H.-J. 1997; Polyamine distribution in actinomycetes with group B peptidoglycan and species of the genera Brevibacterium , Corynebacterium and Tsukamurella . Int J Syst Bacteriol 47:270–277 [CrossRef]
    [Google Scholar]
  3. Buczolits S., Schumann P., Valens M., Rosselló-Mora R., Busse H.-J. 2008; Identification of a bacterial strain isolated from the liver of a laboratory mouse as Microbacterium paraoxydans and emended description of the species Microbacterium paraoxydans Laffineur et al. 2003. Ind J Microbiol 48:243–251 [CrossRef]
    [Google Scholar]
  4. Collins M. D., Jones D., Kroppenstedt R. M. 1983; Reclassification of Brevibacterium imperiale (Steinhaus) and “ Corynebacterium laevaniformans ” (Dias and Bhat) in a redefined genus Microbacterium (Orla-Jensen) as Microbacterium imperiale comb.nov. and Microbacterium laevaniformans nom. rev.; comb. nov. Syst Appl Microbiol 4:65–78 [CrossRef]
    [Google Scholar]
  5. Funke G., Lawson P. A., Nolte F. S., Weiss N., Collins M. D. 1998; Aureobacterium resistens sp. nov., exhibiting vancomycin resistance and teicoplanin susceptibility. FEMS Microbiol Lett 158:89–93 [CrossRef]
    [Google Scholar]
  6. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Kroppenstedt R. M. 2004; Microbacterium benzenivorans sp. nov. Int J Syst Evol Microbiol 54:749–751
    [Google Scholar]
  9. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  10. Kim K. K., Park H. Y., Park W., Kim I. S., Lee S.-T. 2005; Microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm. Int J Syst Evol Microbiol 55:2075–2079
    [Google Scholar]
  11. Li W.-J., Chen H.-H., Kim C.-J., Park D.-J., Tang S.-K., Lee J.-C., Xu L.-H., Jiang C.-L. 2005; Microbacterium halotolerans sp. nov., isolated from a saline soil in the west of China. Int J Syst Evol Microbiol 55:67–70 [CrossRef]
    [Google Scholar]
  12. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  13. Orla-Jensen S. 1919 The Lactic Acid Bacteria Copenhagen: Høst and Son;
    [Google Scholar]
  14. Park H. Y., Kim K. K., Jin L., Lee S.-T. 2006; Microbacterium paludicola sp. nov., a novel xylanolytic bacterium isolated from swamp forest. Int J Syst Evol Microbiol 56:535–539 [CrossRef]
    [Google Scholar]
  15. Rivas R., Trujillo M. E., Sánchez M., Mateos P. F., Martínez-Molina E., Velázquez E. 2004; Microbacterium ulmi sp. nov., a xylanolytic, phosphate-solubilizing bacterium isolated from sawdust of Ulmus nigra . Int J Syst Evol Microbiol 54:513–517
    [Google Scholar]
  16. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  17. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  18. Shivaji S., Bhadra B., Rao S., Chaturvedi P., Kumar Pindi P., Raghukumar C. 2007; Microbacterium indicum sp. nov., isolated from a deep-sea sediment sample from the Chagos Trench. Indian Ocean. Int J Syst Evol Microbiol 57:1819–1822 [CrossRef]
    [Google Scholar]
  19. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576
    [Google Scholar]
  20. Takeuchi M., Hatano K. 1998; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium . Int J Syst Bacteriol 48:739–747 [CrossRef]
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  23. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  24. Young C.-C., Kämpfer P., Shen F.-T., Lai W.-A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
  25. Ziemke F., Höfle M. G., Lalucat J., Rosselló-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
  26. Zlamala C., Schumann P., Kämpfer P., Valens M., Rosselló-Mora R., Lubitz W., Busse H.-J. 2002; Microbacterium aerolatum sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52:1229–1234 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.014092-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014092-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error