1887

Abstract

A novel strictly anaerobic, halophilic and fermentative strain, designated E2R, was isolated from sediments of Xiaokule salt lake in Xinjiang Province, China. Cells were straight to slightly curved, Gram-stain-positive rods that were motile by means of flagella and formed endospores. Strain E2R was moderately halophilic and grew optimally in the presence of 7.5 % NaCl, at pH 8.0 and at 32 °C. Substrates used include yeast extract, Casamino acids, tryptone, fructose, sucrose, xylose, ribose, lactate and tartrate. Thiosulfate could be used as an accessory electron acceptor and stimulated growth. The main fermentation products from fructose were formate and acetate. The predominant fatty acids were iso-C, iso-C F and iso-C. 16S rRNA gene sequence analyses revealed that strain E2R was related most closely to members of the genus (95.5–91.1 % similarity). The G+C content of strain E2R was 28.5 mol%. Strain E2R could be differentiated from its closest relatives based on its halophilic nature and its lower DNA G+C content. It could also be differentiated based on its substrate utilization pattern and relatively high levels of iso-C. On the basis of these data, strain E2R is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E2R (=CGMCC 1.5124 =JCM 16124). An emended description of the genus is also provided.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014084-0
2010-12-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/12/2898.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014084-0&mimeType=html&fmt=ahah

References

  1. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr 25:1324–1328
    [Google Scholar]
  2. Cao X., Liu X., Dong X. 2003; Alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. Int J Syst Evol Microbiol 53:971–975 [CrossRef]
    [Google Scholar]
  3. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Fisher E., Dason A. M., Polshyna G., Lisak J., Crable B., Perera E., Ranganathan M., Thangavelu M., Basu P., Stolz J. F. 2008; Transformation of inorganic and organic arsenic by Alkaliphilus oremlandii sp. nov. strain OhILAs. Ann N Y Acad Sci 1125:230–241 [CrossRef]
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  13. Ollivier B., Caumette P., Garcia J. L., Mah R. A. 1994; Anaerobic bacteria from hypersaline environments. Microbiol Rev 58:27–38
    [Google Scholar]
  14. Oren A. 2006; The order Halobacteriales . . In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology and Biochemistry, 3rd edn. vol 3 pp 113–164 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Takai K., Moser D. P., Onstott T. C., Spoelstra N., Pfiffner S. M., Dohnalkova A., Fredrickson J. K. 2001; Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 51:1245–1256
    [Google Scholar]
  17. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  18. Zhilina T. N., Zavarzina D. G., Kolganova T. V., Lysenko A. M., Tourova T. P. 2009; Alkaliphilus peptidofermentans sp. nov., a new alkaliphilic bacterial soda lake isolate capable of peptide fermentation and Fe(III) reduction. Microbiology [English translation of Mikrobiologiia ] 78:445–454
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.014084-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014084-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error