1887

Abstract

A novel Gram-stain-negative, rod-shaped, non-motile bacterium, strain LW1, was isolated from a water sample collected at a depth of 3.5 m from Lonar Lake, Buldhana district, Maharashtra, India. The cell suspension was reddish-orange due to the presence of carotenoids. Strain LW1 was positive for catalase, oxidase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase, urease and lipase. Fatty acids were dominated by branched-chain fatty acids (>76 %), with a high abundance of iso-C (48 %), anteiso-C (7 %) and iso-C 3-OH (11 %). Strain LW1 contained MK-4 and MK-7 as the major respiratory quinones and phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine as the major phospholipids. A sequence similarity search based on 16S rRNA gene sequences indicated that members of the genera and were the nearest phylogenetic neighbours with similarities of 91.8–92.3 %. Phylogenetic analyses indicated that strain LW1 formed a deep-rooted lineage distinct from the clades represented by the genera , , , and . Based on the above-mentioned phenotypic and phylogenetic characteristics, it is proposed that strain LW1 represents a novel species in a new genus, gen. nov., sp. nov. (type strain LW1=KCTC 22604=CCUG 57479). The genomic DNA G+C content of strain LW1 is 42.7±1 mol%.

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2010-04-01
2020-01-23
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