1887

Abstract

Two actinomycete strains, JSM1-1 and JSM1-3, were isolated from sea sand collected in Thailand. Their taxonomic position was determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus , i.e. the presence of -diaminopimelic acid and glycolyl muramic acid in the peptidoglycan, whole cell sugar pattern D, phospholipids type II, and cellular fatty acid type 3b. Phylogenetic analysis of 16S rRNA gene sequences revealed a close relationship between strains JSM1-1 and JSM1-3 (99.8 %), and between JSM1-1 and JCM 10878 (99.3 %), JCM 3031 (99.0 %), and JCM 13248 (99.0 %). However, strains JSM1-1 and JSM1-3 could be clearly distinguished from these type strains by a low DNA–DNA relatedness and by phenotypic differences. On the basis of the data presented, a new species, sp. nov., is proposed. The type strain is JSM1-1 (=JCM 12870 =PCU 269 =TISTR 1566).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014068-0
2010-03-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/648.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014068-0&mimeType=html&fmt=ahah

References

  1. Ara, I. & Kudo, T. ( 2007; ). Two newspecies of the genus Micromonospora: Micromonospora chokoriensis sp. nov. and Micromonospora coxensis sp. nov., isolated fromsandy soil. J Gen Appl Microbiol 53, 29–37.[CrossRef]
    [Google Scholar]
  2. Arai, T. ( 1975; ). Culture Media forActinomycetes. Tokyo: The Society for Actinomycetes Japan.
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D.E. ( 1977; ). Distribution of menaquinones in actinomycetesand corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridizationin microdilution wells as an alternative to membrane filter hybridizationin which radioisotopes are used to determine genetic relatedness among bacterialstrains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limitson phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang,C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J SystBacteriol 24, 54–63.
    [Google Scholar]
  7. Hirsch, P., Mevs, U., Kroppenstedt, R. M., Schumann, P. &Stackebrandt, E. ( 2004; ). Cryptoendolithic actinomycetesfrom Antarctic sandstone rock samples: Micromonospora endolithicasp. nov. and two isolates related to Micromonospora coerulea Jensen1932. Syst Appl Microbiol 27, 166–174.[CrossRef]
    [Google Scholar]
  8. Hucker, G. J. & Conn, H. J. ( 1923; ). Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93, 3–37.
    [Google Scholar]
  9. Itoh, T., Kudo, T., Parenti, F. & Seino, A. ( 1989; ). Amended description of the genus Kineosporia, basedon chemotaxonomic and morphological studies. Int J Syst Bacteriol 39, 168–173.[CrossRef]
    [Google Scholar]
  10. Jacobson, E., Grauville, W. C. & Fogs, C. E. ( 1958; ). Color Harmony Mannual, 4th edn. Chicago: ContainerCorporation of America.
  11. Jongrungruangchok, S., Tanasupawat, S. & Kudo, T. ( 2008a; ). Micromonospora chaiyaphumensis sp. nov.,isolated from Thai soils. Int J Syst Evol Microbiol 58, 924–928.[CrossRef]
    [Google Scholar]
  12. Jongrungruangchok, S., Tanasupawat, S. & Kudo, T. ( 2008b; ). Micromonospora krabiensis sp. nov., isolatedfrom marine soil in Thailand. J Gen Appl Microbiol 54, 127–133.[CrossRef]
    [Google Scholar]
  13. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteriaand related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  14. Kasai, H., Tamura, T. & Harayama, S. ( 2000; ). Intrageneric relationships among Micromonospora speciesdeduced from gyrB-based phylogeny and DNA relatedness. Int J Syst Bacteriol 50, 127–134.[CrossRef]
    [Google Scholar]
  15. Kawamoto, I. ( 1989; ). Genus Micromonospora Ørskov 1923, 147AL. In Bergey'sManual ofSystematic Bacteriology, vol. 4, pp. 2442–2450. Edited by S. T.Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Kawamoto, I., Oka, T. & Nara, T. ( 1981; ). Cell wall composition of Micromonospora olivasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146, 527–534.
    [Google Scholar]
  17. Kroppenstedt, R. M. ( 1985; ). Fatty acidand menaquinone analysis of actinomycetes and related organisms. In ChemicalMethods in Bacterial Systematics (Society for Applied BacteriologyTechnical Series vol. 20), pp. 173–199. Edited by M. Goodfellow &D. E. Minnikin. New York: Academic Press.
  18. Kroppenstedt, R. M., Mayilraj, S., Wink, J. M., Kallow, W.,Schumann, P., Secondini, C. & Stackebrandt, E. ( 2005; ). Eight new species of the genus Micromonospora, Micromonosporacitrea sp. nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov., Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonosporapeucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov. Syst Appl Microbiol 28, 328–339.[CrossRef]
    [Google Scholar]
  19. Kudo, T., Itoh, T., Miyadoh, S., Shomura, T. & Seino, A. ( 1993; ). Herbidospora gen. nov., a new genusof the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43, 319–328.[CrossRef]
    [Google Scholar]
  20. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary geneticsanalysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  21. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification ofaerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  22. Lechevalier, M. P., De Bievre, C. & Lechevalier, H. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipidcomposition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  23. Mikami, H. & Ishida, Y. ( 1983; ).Post-column fluorometric detection of reducing sugars in high-performanceliquid chromatography using arginine. Bunseki Kagaku 32, E207–E210.[CrossRef]
    [Google Scholar]
  24. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson,G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinonesand polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  25. Nakajima, Y., Kitpreechavanich, V., Suzuki, K. & Kudo, T. ( 1999; ). Microbispora corallina sp. nov.,a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49, 1761–1767.[CrossRef]
    [Google Scholar]
  26. Ørskov, J. ( 1923; ). Investigationsinto the Morphology of the Ray Fungi. Copenhagen: Levin and Munksgaard.
  27. Saitou, N. & Nei, M. ( 1987; ). Theneighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  28. Sasser, M. ( 1990; ). Identification ofbacteria by gas chromatography of cellular fatty acids. MIDI Technical Note101. Newark, DE: MIDI.
  29. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  30. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layerchromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  31. Stevenson, I. L. ( 1967; ). Utilizationof aromatic hydrocarbons by Arthrobacter spp. Can J Microbiol 13, 205–211.[CrossRef]
    [Google Scholar]
  32. Suzuki, K. & Komagata, K. ( 1983; ).Taxonomic significance of cellular fatty acid composition in some coryneformbacteria. Int J Syst Bacteriol 33, 188–200.[CrossRef]
    [Google Scholar]
  33. Tamaoka, J. ( 1994; ). Determination ofDNA base composition. In Chemical Methods in Prokaryotic Systematics,pp. 463–470. Edited by M. Goodfellow & A. G. O'Donnell. Chichester:Wiley.
  34. Tamaoka, J. & Komagata, K. ( 1984; ).Determination of DNA base composition by reversed-phase high-performance liquidchromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  35. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. &Kudo, T. ( 2004; ). Micromonospora aurationigrasp. nov., isolated from a peat swamp forest in Thailand. Actinomycetologica 18, 8–14.[CrossRef]
    [Google Scholar]
  36. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. &Kudo, T. ( 2005a; ). Micromonospora eburneasp. nov., isolated from a Thai peat swamp forest. Int J Syst EvolMicrobiol 55, 417–422.
    [Google Scholar]
  37. Thawai, C., Tanasupawat, S., Itoh, T., Suwanborirux, K. &Kudo, T. ( 2005b; ). Micromonospora siamensissp. nov., isolated from Thai peat swamp forest. J Gen Appl Microbiol 51, 229–234.[CrossRef]
    [Google Scholar]
  38. Thawai, C., Tanasupawat, S., Suwanborirux, K., Itoh, T. &Kudo, T. ( 2008; ). Micromonospora narathiwatensis sp. nov., from Thai peat swamp forest soils. J Gen Appl Microbiol 53, 287–293.
    [Google Scholar]
  39. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. &Higgins, D. G. ( 1997; ). clustal_xwindows interface: flexible strategies for multiple sequence alignment aidedby quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  40. Trujillo, M. E., Fernândez-Molinero, C., Velâzquez,E., Kroppenstedt, R. M., Schumann, P., Mateos, P. F. & Martínez-Molina,E. ( 2005; ). Micromonospora mirobrigensis sp.nov. Int J Syst Evol Microbiol 55, 877–880.[CrossRef]
    [Google Scholar]
  41. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P., Carro, L. &Martínez-Molina, E. ( 2006; ). Micromonosporacoriariae sp. nov., isolated from root nodules of Coriaria myrtifolia. Int J Syst Evol Microbiol 56, 2381–2385.[CrossRef]
    [Google Scholar]
  42. Trujillo, M. E., Kroppenstedt, R. M., Fernândez-Molinero,C., Schumann, P. & Martínez-Molina, E. ( 2007; ). Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius. Int J Syst Evol Microbiol 57, 2799–2804.[CrossRef]
    [Google Scholar]
  43. Uchida, K. & Aida, K. ( 1984; ). Animproved method for the glycolate test for simple identification of the acyltype of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  44. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A.D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray,R. G. E. & other authors ( 1987; ). InternationalCommittee on Systematic Bacteriology. Report of the ad hoc committee on thereconciliation of approaches to bacterial systematics. Int J SystBacteriol 37, 463–464.
    [Google Scholar]
  45. Williams, S. T. & Cross, T. ( 1971; ).Actinomycetes. In Methods in Microbiology, vol. 4, pp. 295–334.Edited by C. Booth. London: Academic Press
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.014068-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014068-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 648 - 652

IMAGE

Maximum-parsimony tree based on almost complete 16S rRNA gene sequences [ PDF] (16 KB)

PDF

PDF of Supplementary Tables 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error