1887

Abstract

Two actinomycete strains, JSM1-1 and JSM1-3, were isolated from sea sand collected in Thailand. Their taxonomic position was determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the genus , i.e. the presence of -diaminopimelic acid and glycolyl muramic acid in the peptidoglycan, whole cell sugar pattern D, phospholipids type II, and cellular fatty acid type 3b. Phylogenetic analysis of 16S rRNA gene sequences revealed a close relationship between strains JSM1-1 and JSM1-3 (99.8 %), and between JSM1-1 and JCM 10878 (99.3 %), JCM 3031 (99.0 %), and JCM 13248 (99.0 %). However, strains JSM1-1 and JSM1-3 could be clearly distinguished from these type strains by a low DNA–DNA relatedness and by phenotypic differences. On the basis of the data presented, a new species, sp. nov., is proposed. The type strain is JSM1-1 (=JCM 12870 =PCU 269 =TISTR 1566).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014068-0
2010-03-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/648.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014068-0&mimeType=html&fmt=ahah

References

  1. Ara I., Kudo T. 2007; Two new species of the genus Micromonospora : Micromonospora chokoriensis sp.nov. and Micromonospora coxensis sp. nov., isolated from sandy soil. J Gen Appl Microbiol 53:29–37 [CrossRef]
    [Google Scholar]
  2. Arai T. 1975 Culture Media for Actinomycetes Tokyo: The Society for Actinomycetes Japan;
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63
    [Google Scholar]
  7. Hirsch P., Mevs U., Kroppenstedt R. M., Schumann P., Stackebrandt E. 2004; Cryptoendolithic actinomycetes from Antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932. Syst Appl Microbiol 27:166–174 [CrossRef]
    [Google Scholar]
  8. Hucker G. J., Conn H. J. 1923; Method of Gram staining. N Y State Agric Exp Stn Tech Bull 93:3–37
    [Google Scholar]
  9. Itoh T., Kudo T., Parenti F., Seino A. 1989; Amended description of the genus Kineosporia , based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [CrossRef]
    [Google Scholar]
  10. Jacobson E., Grauville W. C., Fogs C. E. 1958 Color Harmony Mannual , 4th edn. Chicago: Container Corporation of America;
    [Google Scholar]
  11. Jongrungruangchok S., Tanasupawat S., Kudo T. 2008a; Micromonospora chaiyaphumensis sp. nov., isolated from Thai soils. Int J Syst Evol Microbiol 58:924–928 [CrossRef]
    [Google Scholar]
  12. Jongrungruangchok S., Tanasupawat S., Kudo T. 2008b; Micromonospora krabiensis sp. nov., isolated from marine soil in Thailand. J Gen Appl Microbiol 54:127–133 [CrossRef]
    [Google Scholar]
  13. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  14. Kasai H., Tamura T., Harayama S. 2000; Intrageneric relationships among Micromonospora species deduced from gyrB -based phylogeny and DNA relatedness. Int J Syst Bacteriol 50:127–134 [CrossRef]
    [Google Scholar]
  15. Kawamoto I. 1989; Genus Micromonospora Ørskov 1923, 147AL . In Bergey'sManual of Systematic Bacteriology vol 4 pp 2442–2450 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivasterospora , Micromonospora sagamiensis , and related organisms. J Bacteriol 146:527–534
    [Google Scholar]
  17. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20) pp 173–199 Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;
    [Google Scholar]
  18. Kroppenstedt R. M., Mayilraj S., Wink J. M., Kallow W., Schumann P., Secondini C., Stackebrandt E. 2005; Eight new species of the genus Micromonospora , Micromonospora citrea sp.nov., Micromonospora echinaurantiaca sp. nov., Micromonospora echinofusca sp. nov.,Micromonospora fulviviridis sp. nov., Micromonospora inyonensis sp. nov., Micromonospora peucetia sp. nov., Micromonospora sagamiensis sp. nov., and Micromonospora viridifaciens sp. nov. Syst Appl Microbiol 28:328–339 [CrossRef]
    [Google Scholar]
  19. Kudo T., Itoh T., Miyadoh S., Shomura T., Seino A. 1993; Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43:319–328 [CrossRef]
    [Google Scholar]
  20. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  21. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  22. Lechevalier M. P., De Bievre C., Lechevalier H. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  23. Mikami H., Ishida Y. 1983; Post-column fluorometric detection of reducing sugars in high-performance liquid chromatography using arginine. Bunseki Kagaku 32:E207–E210 [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  25. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. 1999; Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49:1761–1767 [CrossRef]
    [Google Scholar]
  26. Ørskov J. 1923 Investigations into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard;
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  30. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  31. Stevenson I. L. 1967; Utilization of aromatic hydrocarbons by Arthrobacter spp. Can J Microbiol 13:205–211 [CrossRef]
    [Google Scholar]
  32. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33:188–200 [CrossRef]
    [Google Scholar]
  33. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp 463–470 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  34. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  35. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. 2004; Micromonospora aurationigra sp. nov., isolated from a peat swamp forest in Thailand. Actinomycetologica 18:8–14 [CrossRef]
    [Google Scholar]
  36. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. 2005a; Micromonospora eburnea sp. nov., isolated from a Thai peat swamp forest. Int J Syst Evol Microbiol 55:417–422
    [Google Scholar]
  37. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. 2005b; Micromonospora siamensis sp. nov., isolated from Thai peat swamp forest. J Gen Appl Microbiol 51:229–234 [CrossRef]
    [Google Scholar]
  38. Thawai C., Tanasupawat S., Suwanborirux K., Itoh T., Kudo T. 2008; Micromonospora narathiwatensis sp. nov., from Thai peat swamp forest soils. J Gen Appl Microbiol 53:287–293
    [Google Scholar]
  39. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  40. Trujillo M. E., Fernândez-Molinero C., Velâzquez E., Kroppenstedt R. M., Schumann P., Mateos P. F., Martínez-Molina E. 2005; Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55:877–880 [CrossRef]
    [Google Scholar]
  41. Trujillo M. E., Kroppenstedt R. M., Schumann P., Carro L., Martínez-Molina E. 2006; Micromonospora coriariae sp. nov., isolated from root nodules of Coriaria myrtifolia . Int J Syst Evol Microbiol 56:2381–2385 [CrossRef]
    [Google Scholar]
  42. Trujillo M. E., Kroppenstedt R. M., Fernândez-Molinero C., Schumann P., Martínez-Molina E. 2007; Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius . Int J Syst Evol Microbiol 57:2799–2804 [CrossRef]
    [Google Scholar]
  43. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  44. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on the reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
  45. Williams S. T., Cross T. 1971).Actinomycetes In Methods in Microbiology vol 4 pp 295–334 Edited by Booth C. London: Academic Press;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.014068-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014068-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error