sp. nov., isolated from a sulfur spring Free

Abstract

A novel bacterium, designated strain HT27, was isolated from a sulfur spring sample collected from Athamallik, Orissa, India, and was characterized by using a polyphasic approach. Cells were Gram-negative, strictly aerobic, rod-shaped and motile by means of a single polar flagellum. Strain HT27 was oxidase- and catalase-positive. Growth was observed at pH 5.0–11.0 and at 15–45 °C; the highest growth yield was observed at pH 7.5–8.0 and 30–37 °C. The G+C content of the genomic DNA of strain HT27 was 63 mol%. The major cellular fatty acids were C 7 (44.24 %), C (27.65 %), C 7 (13.98 %), C (2.60 %) and C 3-OH (2.22 %). 16S rRNA gene sequence analysis indicated that strain HT27 clustered with the genus and showed 99.0 % similarity to E4FC31. However, the level of DNA–DNA relatedness between strain HT27 and E4FC31 was 30 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridization data, strain HT27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT27 (=DSM 17901 =JCM 15969).

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2010-05-01
2024-03-29
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Bhadra B., Raghukumar C., Pindi P. K., Shivaji S. 2008; Brevibacterium oceani sp. nov., isolated from deep-sea sediment of the Chagos Trench. Indian Ocean. Int J Syst Evol Microbiol 58:57–60 [CrossRef]
    [Google Scholar]
  3. Das S. K., Mishra A. K., Tindall B. J., Rainey F. A., Stackebrandt E. 1996; Oxidation of thiosulfate by a new bacterium, Bosea thiooxidans (strain BI-42) gen. nov., sp. nov.: analysis of phylogeny based on chemotaxonomy and 16S ribosomal DNA sequencing. Int J Syst Bacteriol 46:981–987 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Kuykendall L. D., Roy M. D., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  9. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Panda S. K., Jyoti V., Bhadra B., Nayak K. C., Shivaji S., Rainey F. A., Das S. K. 2009; Thiomonas bhubaneswarensis sp. nov., a novel obligately mixotrophic, moderately thermophilic, thiosulfate-oxidizing bacterium. Int J Syst Evol Microbiol 59:2171–2175 [CrossRef]
    [Google Scholar]
  12. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Sharma D. P., Thomas C., Hall R. H., Levine M. M., Attridge S. R. 1989; Significance of toxin-coregulated pili as protective antigens of Vibrio cholerae in the infant mouse model. Vaccine 7:451–456 [CrossRef]
    [Google Scholar]
  14. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  15. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  16. Vaz-Moreira I., Nobre M. F., Nunes O. C., Manaia C. M. 2007; Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 57:1108–1112 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Good R. C., Krichevsky M. I., Blacklock Z., David H. L., Dawson D., Gross W., Hawkins J., Levy-Frebault V. V. other authors 1991; Fourth report of the cooperative, open-ended study of slowly growing mycobacteria by the International Working Group on Mycobacterial Taxonomy. Int J Syst Bacteriol 41:463–472 [CrossRef]
    [Google Scholar]
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