1887

Abstract

A novel bacterium, designated strain HT27, was isolated from a sulfur spring sample collected from Athamallik, Orissa, India, and was characterized by using a polyphasic approach. Cells were Gram-negative, strictly aerobic, rod-shaped and motile by means of a single polar flagellum. Strain HT27 was oxidase- and catalase-positive. Growth was observed at pH 5.0–11.0 and at 15–45 °C; the highest growth yield was observed at pH 7.5–8.0 and 30–37 °C. The G+C content of the genomic DNA of strain HT27 was 63 mol%. The major cellular fatty acids were C 7 (44.24 %), C (27.65 %), C 7 (13.98 %), C (2.60 %) and C 3-OH (2.22 %). 16S rRNA gene sequence analysis indicated that strain HT27 clustered with the genus and showed 99.0 % similarity to E4FC31. However, the level of DNA–DNA relatedness between strain HT27 and E4FC31 was 30 %. On the basis of phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequence analysis and DNA–DNA hybridization data, strain HT27 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT27 (=DSM 17901 =JCM 15969).

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2010-05-01
2019-10-14
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