1887

Abstract

A novel Gram-negative, orange-pigmented, slightly halophilic, rod-shaped bacterium, strain T30, was isolated from sediment from the South China Sea. Phylogenetic analysis showed that strain T30 was a member of the genus , sharing highest 16S rRNA gene sequence similarities with JCM 12189 (99.5 %) and DSM 17792 (99.0 %). Levels of DNA–DNA relatedness between strain T30 and closely related strains of species ranged from 14.5 to 56.9 %.The isolate lacked bacteriochlorophyll and contained ubiquinone-10 as the predominant respiratory lipoquinone. The major fatty acids of this strain were C 7 (38.2 %) and C 7 /C 6 (17.4 %). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and phosphatidylglycerol. The DNA G+C content of strain T30 was 59.5 mol%. On the basis of phenotypic and phylogenetic data, a novel species, sp. nov., is proposed; the type strain is T30 (=CGMCC 1.7715=JCM 16125).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.014027-0
2010-09-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/9/2215.html?itemId=/content/journal/ijsem/10.1099/ijs.0.014027-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J. Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Denner, E. B. M., Vybiral, D., Koblížek, M., Kämpfer, P., Busse, H.-J. & Velimirov, B. ( 2002; ). Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea. Int J Syst Evol Microbiol 52, 1655–1661.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  6. Huelsenbeck, J. P. & Ronquist, F. ( 2001; ). mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.[CrossRef]
    [Google Scholar]
  7. Ivanova, E. P., Bowman, J. P., Lysenko, A. M., Zhukova, N. V., Gorshkova, N. M., Kuznetsova, T. A., Kalinovskaya, N. I., Shevchenko, L. S. & Mikhailov, V. V. ( 2005; ). Erythrobacter vulgaris sp. nov., a novel organism isolated from the marine invertebrates. Syst Appl Microbiol 28, 123–130.[CrossRef]
    [Google Scholar]
  8. Kates, M. ( 1986; ). Techniques of Lipidology, 2nd edn. Amsterdam. : Elsevier.
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt, E. & Goodfellow, M.. Chichester. : Wiley.
    [Google Scholar]
  12. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Reichenbach, H. ( 1992; ). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by Balows, A., Trüper, H. G., Dworkin, M., Harder, W. & Schleifer, K. H.. New York. : Springer.
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Shiba, T. & Simidu, U. ( 1982; ). Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32, 211–217.[CrossRef]
    [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Phillips, G. B.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  19. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  20. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  21. Yoon, J. H., Kim, H., Kim, I. G., Kang, K. H. & Park, Y. H. ( 2003; ). Erythrobacter flavus sp. nov., a slight halophile from the East Sea in Korea. Int J Syst Evol Microbiol 53, 1169–1174.[CrossRef]
    [Google Scholar]
  22. Yoon, J. H., Kang, K. H., Oh, T. K. & Park, Y. H. ( 2004; ). Erythrobacter aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1981–1985.[CrossRef]
    [Google Scholar]
  23. Yoon, J. H., Oh, T. K. & Park, Y. H. ( 2005; ). Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 71–75.[CrossRef]
    [Google Scholar]
  24. Yurkov, V., Stackebrandt, E., Holmes, A., Fuerst, J. A., Hugenholtz, P., Golecki, J., Gad'on, N., Gorlenko, V. M., Kompantseva, E. I. & Drews, G. ( 1994; ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44, 427–434.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.014027-0
Loading
/content/journal/ijsem/10.1099/ijs.0.014027-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2215–2220

Maximum-likelihood tree based on the 16S rRNA gene sequences of strain T30 and closely related species [ PDF] (218 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error