1887

Abstract

A thermophilic, anaerobic, chemosynthetic bacterium, designated strain MB-1, was isolated from the walls of an active deep-sea hydrothermal vent chimney on the East Pacific Rise at  ° 50′ N 10 ° 17′ W. The cells were Gram-negative-staining rods, approximately 1–1.5 μm long and 0.3–0.5 μm wide. Strain MB-1 grew at 25–65 °C (optimum 55 °C), with 10–35 g NaCl l (optimum 20 g l) and at pH 4.5–8.5 (optimum pH 7.0). Generation time under optimal conditions was 45.6 min. Growth occurred under chemolithoautotrophic conditions with H as the energy source and CO as the carbon source. Nitrate was used as the electron acceptor, with resulting production of ammonium. Thiosulfate, sulfur and selenate were also used as electron acceptors. No growth was observed in the presence of lactate, peptone or tryptone. Chemo-organotrophic growth occurred in the presence of acetate, formate, Casamino acids, sucrose, galactose and yeast extract under a N/CO gas phase. The G+C content of the genomic DNA was 36.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to AmH, PH1209 and 525 (95, 94 and 93 % sequence identity, respectively). On the basis of phylogenetic, physiological and genetic considerations, it is proposed that the organism represents a novel species within the genus , sp. nov. The type strain is MB-1 (=DSM 22087 =JCM 15746).

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2010-05-01
2024-03-28
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