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Abstract

A novel actinomycete strain, designated MS498, was isolated from an ocean sediment sample collected from the South China Sea. It was subjected to a polyphasic analysis to determine its taxonomic position. The phylogenetic tree based on 16S rRNA gene sequences showed that strain MS498 had the highest similarity (96.5 %) with members of the genus and was loosely associated with DSM 43194 and DSM 44411. Based on 16S rRNA gene sequence analysis, phenotypic characteristics and chemotaxonomic data, the new isolate is proposed to represent a novel species of the genus , named sp. nov. (type strain MS498=CCTCC AA 208056 =DSM 45268).

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2010-04-01
2019-10-20
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vol. , part 4, pp. 985 - 989

Scanning electron micrograph showing the spore chain morphology and fragmented substrate mycelia of strain MS498 grown on ISP 2 medium for 14 days at 28 °C.

Cultural characteristics of strain MS498 and its closest relative, . The type strain of was received from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ; Braunschweig, Germany). All media for DSM 43194 were supplemented with 10% NaCl (w/v, pH 7.2). After 14 days of incubation at 28 °C, both strains showed good growth on Czapek agar, oatmeal agar (ISP 3), potato agar and nutrient agar medium. ISP, International Streptomyces project (Shirling & Gottlieb, 1966). Colours are taken from the ISCC-NBS colour charts (Kelly, 1964).

Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.

Methods for characterization of species. , 313–340.



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