1887

Abstract

During a study of the prevalence of in black-headed gulls () in Sweden, three isolates, strains LMG 24379, LMG 24380 and LMG 24381, were initially identified as . Further characterization by both AFLP and whole-cell protein SDS-PAGE analyses revealed that they formed a distinct group in the genus . This unique position was confirmed by phenotypic characterization, 16S rRNA and gene sequence analysis and DNA–DNA hybridizations. The combined data confirm that these isolates represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is LMG 24380 (=CCUG 57498).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013748-0
2010-08-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/8/1870.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013748-0&mimeType=html&fmt=ahah

References

  1. Broman, T., Palmgren, H., Bergstrom, S., Sellin, M., Waldenstrom, J., Danielsson-Tham, M. L. & Olsen, B. ( 2002; ). Campylobacter jejuni in black-headed gulls (Larus ridibundus): prevalence, genotypes, and influence on C. jejuni epidemiology. J Clin Microbiol 40, 4594–4602.[CrossRef]
    [Google Scholar]
  2. Broman, T., Waldenstrom, J., Dahlgren, D., Carlsson, I., Eliasson, I. & Olsen, B. ( 2004; ). Diversities and similarities in PFGE profiles of Campylobacter jejuni isolated from migrating birds and humans. J Appl Microbiol 96, 834–843.[CrossRef]
    [Google Scholar]
  3. Colles, F. M., McCarthy, N. D., Howe, J. C., Devereux, C. L., Gosler, A. G. & Maiden, M. C. ( 2009; ). Dynamics of Campylobacter colonization of a natural host, Sturnus vulgaris (European starling). Environ Microbiol 11, 258–267.[CrossRef]
    [Google Scholar]
  4. Debruyne, L., Gevers, D. & Vandamme, P. ( 2008; ). Taxonomy of the family Campylobacteraceae. In Campylobacter, pp. 3–26. Edited by I. Nachamkin, C. M. Szymanski & M. J. Blaser. Washington, DC: American Society for Microbiology.
  5. Debruyne, L., On, S. L. W., De Brandt, E. & Vandamme, P. ( 2009; ). Novel Campylobacter lari-like bacteria from humans and molluscs: description of Campylobacter peloridis sp. nov., Campylobacter lari subsp. concheus subsp. nov. and Campylobacter lari subsp. lari subsp. nov. Int J Syst Evol Microbiol 59, 1126–1132.[CrossRef]
    [Google Scholar]
  6. Debruyne, L., Broman, T., Bergström, S., Olsen, B., On, S. L. W. & Vandamme, P. ( 2010; ). Campylobacter subantarcticus sp. nov., isolated from birds in the sub-Antarctic region. Int J Syst Evol Microbiol 60, 815–819.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Fermer, C. & Engvall, E. O. ( 1999; ). Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. J Clin Microbiol 37, 3370–3373.
    [Google Scholar]
  9. Foster, G., Holmes, B., Steigerwalt, A. G., Lawson, P. A., Thorne, P., Byrer, D. E., Ross, H. M., Xerry, J., Thompson, P. M. & Collins, M. D. ( 2004; ). Campylobacter insulaenigrae sp. nov., isolated from marine mammals. Int J Syst Evol Microbiol 54, 2369–2373.[CrossRef]
    [Google Scholar]
  10. French, N. P., Midwinter, A., Holland, B., Collins-Emerson, J., Pattison, R., Colles, F. & Carter, P. ( 2009; ). Molecular epidemiology of Campylobacter jejuni isolates from wild-bird fecal material in children's playgrounds. Appl Environ Microbiol 75, 779–783.[CrossRef]
    [Google Scholar]
  11. Inglis, G. D., Hoar, B. M., Whiteside, D. P. & Morck, D. W. ( 2007; ). Campylobacter canadensis sp. nov., from captive whooping cranes in Canada. Int J Syst Evol Microbiol 57, 2636–2644.[CrossRef]
    [Google Scholar]
  12. Kärenlampi, R. I., Tolvanen, T. P. & Hanninen, M. L. ( 2004; ). Phylogenetic analysis and PCR-restriction fragment length polymorphism identification of Campylobacter species based on partial groEL gene sequences. J Clin Microbiol 42, 5731–5738.[CrossRef]
    [Google Scholar]
  13. Logan, J. M. J., Burnens, A., Linton, D., Lawson, A. J. & Stanley, J. ( 2000; ). Campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir. Int J Syst Evol Microbiol 50, 865–872.[CrossRef]
    [Google Scholar]
  14. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  15. On, S. L. W., Holmes, B. & Sackin, M. J. ( 1996; ). A probability matrix for the identification of campylobacters, helicobacters and allied taxa. J Appl Bacteriol 81, 425–432.
    [Google Scholar]
  16. On, S. L. W., Atabay, H. I., Corry, J. E. L., Harrington, C. S. & Vandamme, P. ( 1998; ). Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans. Int J Syst Bacteriol 48, 195–206.[CrossRef]
    [Google Scholar]
  17. Palmgren, H., Broman, T., Waldenstrom, J., Lindberg, P., Aspan, A. & Olsen, B. ( 2004; ). Salmonella Amager, Campylobacter jejuni, and urease-positive thermophilic Campylobacter found in free-flying peregrine falcons (Falco peregrinus) in Sweden. J Wildl Dis 40, 583–587.[CrossRef]
    [Google Scholar]
  18. Petersen, L., Nielsen, E. M., Engberg, J., On, S. L. & Dietz, H. H. ( 2001; ). Comparison of genotypes and serotypes of Campylobacter jejuni isolated from Danish wild mammals and birds and from broiler flocks and humans. Appl Environ Microbiol 67, 3115–3121.[CrossRef]
    [Google Scholar]
  19. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  20. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  21. Rossi, M., Debruyne, L., Zanoni, R. G., Manfreda, G., Revez, J. & Vandamme, P. ( 2009; ). Campylobacter avium sp. nov., a hippurate-positive species isolated from poultry. Int J Syst Evol Microbiol 59, 2364–2369.[CrossRef]
    [Google Scholar]
  22. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  23. Sebald, M. & Véron, M. ( 1963; ). Base DNA content and classification of vibrios. Ann Inst Pasteur (Paris) 105, 897–910.
    [Google Scholar]
  24. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  26. Vandamme, P., Pot, B., Falsen, E., Kersters, K. & De Ley, J. ( 1990; ). Intra- and interspecific relationships of veterinary campylobacters revealed by numerical analysis of electrophoretic protein profiles and DNA : DNA hybridizations. Syst Appl Microbiol 13, 295–303.[CrossRef]
    [Google Scholar]
  27. Vandamme, P., Falsen, E., Rossau, R., Hoste, B., Segers, P., Tytgat, P. & De Ley, J. ( 1991; ). Revision of Campylobacter, Helicobacter and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41, 88–103.[CrossRef]
    [Google Scholar]
  28. Vandamme, P., Van Doorn, L.-J., al Rashid, S. T., Quint, W. G. V., van der Plas, J., Chan, V. L. & On, S. L. W. ( 1997; ). Campylobacter hyoilei Alderton et al. 1995 and Campylobacter coli Véron and Chatelain 1973 are subjective synonyms. Int J Syst Bacteriol 47, 1055–1060.[CrossRef]
    [Google Scholar]
  29. Vandamme, P., Holmes, B., Bercovier, H. & Coenye, T. ( 2006; ). Classification of Centers for Disease Control Group Eugonic Fermenter (EF)-4a and EF-4b as Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov., respectively. Int J Syst Evol Microbiol 56, 1801–1805.[CrossRef]
    [Google Scholar]
  30. Waldenström, J., Broman, T., Carlsson, I., Hasselquist, D., Achterberg, R. P., Wagenaar, J. A. & Olsen, B. ( 2002; ). Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Appl Environ Microbiol 68, 5911–5917.[CrossRef]
    [Google Scholar]
  31. Waldenström, J., On, S. L., Ottvall, R., Hasselquist, D. & Olsen, B. ( 2007; ). Species diversity of campylobacteria in a wild bird community in Sweden. J Appl Microbiol 102, 424–432.
    [Google Scholar]
  32. Zanoni, R. G., Debruyne, L., Rossi, M., Revez, J. & Vandamme, P. ( 2009; ). Campylobacter cuniculorum sp. nov., from rabbits. Int J Syst Evol Microbiol 59, 1666–1671.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013748-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013748-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1870 - 1875

Dendrogram representing numerical comparison of AFLP fingerprints of three strains representing sp. nov. and selected reference strains.

UPGMA dendrogram of partial whole-cell protein SDS-PAGE profiles.

Differentiating characteristics between the novel strains and an extended selection of species.

[PDF file of Supplementary Figures and Table](187 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error