1887

Abstract

A methylotrophic nitrogen-fixing bacterial strain, Ah-143, isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis combined with gene sequence analysis allocated strain Ah-143 to the family , with and as the closest relatives. The strain is Gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shaped cells with a DNA GC content of approximately 53.2 mol%. The strain utilizes methanol as a carbon source and the gene was closely related to the gene of members of the genus . The fatty acid profile consisted of C, C cyclo, C 7, summed feature 2 (iso-C I and/or C 3-OH) and summed feature 3 (iso-C 2-OH and/or C 7) as the major components. DNA–DNA relatedness of strain Ah-143 with its close relatives was less than 20 %. On the basis of the phylogenetic analyses, DNA–DNA hybridization data, and unique physiological and biochemical characteristics, it is proposed that the strain represents a novel species of the genus and should be named sp. nov. The type strain is Ah-143 (=NCIMB 14469 =KCTC 22375).

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2010-07-01
2021-04-16
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