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Abstract

A Gram-stain-positive, oxidase- and catalase-positive, non-motile, non-spore-forming, halotolerant, coccoid bacterium, designated strain NY-2, was isolated from a seaside soil sample from Shandong Province, China. Strain NY-2 was able to grow in the presence of 0–23 % (w/v) NaCl and at pH 4.5–10.0 and 5–42 °C; optimum growth was observed with 3–8 % (w/v) total salts and at pH 6.5–8.0 and 28–37 °C. Chemotaxonomic analyses, including fatty acid profiles, menaquinones and polar lipids, supported the affiliation of strain NY-2 to the genus . The predominant menaquinone of strain NY-2 was menaquinone 7 (MK-7) (100 %) and the major cellular fatty acids were iso-C (49.0 %) and anteiso-C (19.6 %). Cellular polar lipids were phosphatidylglycerol, diphosphatidylglycerol and several unidentified phospholipids. The DNA G+C content of strain NY-2 was 36.8 mol%. Based on 16S rRNA gene sequence analysis, strain NY-2 formed a coherent cluster with JSM 076033, YKJ-101 and YKJ-115. Phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data indicated that strain NY-2 (=CCTCC AB 208288 =JCM 15687) should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2010-06-01
2021-05-16
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