1887

Abstract

Two aerobic, Gram-positive, rod-shaped bacterial strains, 5YN10-14 and GR21-5, were isolated from the Yongneup wetland and ginseng soil in Korea, respectively. The two strains formed ellipsoidal or oval spores positioned centrally or paracentrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, these strains were related to members of the genus . 16S rRNA gene sequence similarity between strains 5YN10-14 and GR21-5 was 95.9 %. Strains 5YN10-14 and GR21-5 showed, respectively, 94.3 and 95.2 % 16S rRNA gene sequence similarity to CCUG 47242, 94.6 and 94.4 % to HKU3, 94.7 and 94.7 % to RI-39, and 95.4 and 94.8 % to GSPC1. The major fatty acids of strain 5YN10-14 were anteiso-C (51.1 %), iso-C (18.5 %) and C (13.2 %), and the major fatty acids of strain GR21-5 were anteiso-C (48.9 %), iso-C (15.0 %) and iso-C (12.2 %). The two strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids; however, strain 5YN10-14 also contained lysylphosphatidylglycerol as a major polar lipid, whereas strain GR21-5 had an unknown aminophospholipid as another major polar lipid. The DNA G+C contents of strains 5YN10-14 and GR21-5 were 58.8 and 61.3 mol%, respectively. Based on the results of the phylogenetic and phenotypic data presented, it was concluded that the two strains represent two novel species of the genus , for which the names sp. nov. (type strain 5YN10-14=KACC 11768=DSM 18998) and sp. nov. (type strain GR21-5=KACC 11771=DSM 18997) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013581-0
2010-03-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/526.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013581-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman,J. G., Smith, J. A. & Struhl, K. (editors) ( 1987; ). Current Protocols in Molecular Biology. New York: Wiley.
  2. Cho, E. A., Lee, J. S., Lee, K. C., Jung, H. C., Pan, J. G. &Pyun, Y. R. ( 2007; ). Cohnella laeviribosisp. nov., isolated from a volcanic pond. Int J Syst Evol Microbiol 57, 2902–2907.[CrossRef]
    [Google Scholar]
  3. García-Fraile, P., Velázquez, E., Mateos, P. F.,Martínez-Molina, E. & Rivas, R. ( 2008; ). Cohnella phaseoli sp. nov., isolated from root nodules of Phaseoluscoccineus in Spain, and emended description of the genus Cohnella. Int J Syst Evol Microbiol 58, 1855–1859.[CrossRef]
    [Google Scholar]
  4. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey,F. A. ( 1996; ). Agrococcus jenensis gen. nov.,sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cellwall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  5. Kämpfer, P., Rosselló-Mora, R., Falsen, E., Busse,H. J. & Tindall, B. J. ( 2006; ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillushongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56, 781–786.[CrossRef]
    [Google Scholar]
  6. Kimura, M. ( 1983; ). The Neutral Theoryof Molecular Evolution. Cambridge: Cambridge University Press.
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary geneticsanalysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleicacid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  9. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson,G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinonesand polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  10. Reasoner, D. J. & Geldreich, E. E. ( 1985; ). A new medium for the enumeration and subculture of bacteria from potablewater. Appl Environ Microbiol 49, 1–7.
    [Google Scholar]
  11. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and MolecularBacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray,W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  12. Teng, J. L., Woo, P. C., Leung, K. W., Lau, S. K., Wong, M.K. & Yuen, K. Y. ( 2003; ). Pseudobacteraemia ina patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov. Mol Pathol 56, 29–35.[CrossRef]
    [Google Scholar]
  13. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivityof progressive multiple sequence alignment through sequence weighting, position-specificgap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  14. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A.D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray,R. G. E. & other authors ( 1987; ). InternationalCommittee on Systematic Bacteriology. Report of the ad hoc committee on reconciliationof approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  15. Weon, H.-Y., Kim, B.-Y., Kwon, S.-W., Park, I.-C., Cha, I.-B.,Tindall, B. J., Stackebrandt, E., Trüper, H. G. & Go, S.-J. ( 2005; ). Leadbetterella byssophila gen. nov., sp.nov., isolated from cotton-waste composts for the cultivation of oyster mushroom. Int J Syst Evol Microbiol 55, 2297–2302.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013581-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013581-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 526 - 530

 



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error