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Abstract

A Gram-stain-positive, coccoid, non-endospore-forming actinobacterium (strain CC-12602) was isolated from a spawn used for growing the edible mushroom in the laboratory. On the basis of 16S rRNA gene sequence analysis, strain CC-12602 was shown to belong to the genus and was related most closely to the type strains of (96.1 % similarity), (95.9 %), (95.8 %) and (95.5 %). The quinone system comprised menaquinone MK-9(H) as the major component and the polyamine pattern consisted of spermidine and spermine as major compounds. The predominant polar lipids were phosphatidylglycerol and unknown phospholipid PL3. Moderate amounts of diphosphatidylglycerol, an unknown glycolipid and three unknown phospholipids and minor amounts of an unknown phospholipid and a polar lipid were detected. The peptidoglycan type was A3′, based on -2,6-diaminopimelic acid with an interpeptide bridge consisting of a single glycine residue and a second glycine residue at position 1 of the peptide subunit. Peptidoglycan structure and major fatty acids (anteiso-C, iso-C and iso-C) supported the affiliation of strain CC-12602 to the genus . The results of physiological and biochemical tests allowed strain CC-12602 to be differentiated phenotypically from recognized species. Strain CC-12602 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CC-12602 (=DSM 21800 =CCM 7685).

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2010-04-01
2024-03-29
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [CrossRef]
    [Google Scholar]
  2. Altenburger P., Kämpfer P., Akimov V. N., Lubitz W., Busse H.-J. 1997; Polyamine distribution in actinomycetes with group B peptidoglycan and species of the genera Brevibacterium , Corynebacterium and Tsukamurella . Int J Syst Bacteriol 47:270–277 [CrossRef]
    [Google Scholar]
  3. An D.-S., Im W.-T., Yoon M.-H. 2008; Microlunatus panaciterrae sp. nov., a β -glucosidase-producing bacterium, isolated from soil in a ginseng field. Int J Syst Evol Microbiol 58:2734–2738
    [Google Scholar]
  4. Busse H.-J., Schumann P. 1999; Polyamine profiles within genera of the class Actinobacteria with ll-diaminopimelic acid in the peptidoglycan. Int J Syst Bacteriol 49:179–184
    [Google Scholar]
  5. Cui Y. S., Im W. T., Yin C. R., Yang D. C., Lee S. T. 2007; Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 57:713–716 [CrossRef]
    [Google Scholar]
  6. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  7. Hamana K. 1995; Polyamine distribution patterns in coryneform bacteria and related gram-positive eubacteria. Annu Rep Coll Med Care Technol Gunma Univ 16:69–77
    [Google Scholar]
  8. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 2004; Pseudonocardia benzenivorans sp. nov. Int J Syst Evol Microbiol 54:749–751
    [Google Scholar]
  10. Kämpfer P., Steiof M., Dott W. 1991; Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Lodders N., Warfolomeow I., Busse H.-J. 2009; Tessaracoccus lubricantis sp. nov., isolated from a metalworking fluid. Int J Syst Evol Microbiol 59:1545–1549 [CrossRef]
    [Google Scholar]
  12. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  13. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Kamagata Y. 1995; Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Bacteriol 45:17–22 [CrossRef]
    [Google Scholar]
  14. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  15. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  16. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov., an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [CrossRef]
    [Google Scholar]
  17. Stolz A., Busse H.-J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  19. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  20. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [CrossRef]
    [Google Scholar]
  21. Wang Y.-X., Cai M., Zhi X.-Y., Zhang Y.-Q., Tang S.-K., Yu L.-H., Cui X.-L., Li W.-J. 2008; Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample. Int J Syst Evol Microbiol 58:1873–1877 [CrossRef]
    [Google Scholar]
  22. Young C.-C., Kämpfer P., Shen F.-T., Lai W.-A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
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