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Abstract

A Gram-stain-positive, coccoid, non-endospore-forming actinobacterium (strain CC-12602) was isolated from a spawn used for growing the edible mushroom in the laboratory. On the basis of 16S rRNA gene sequence analysis, strain CC-12602 was shown to belong to the genus and was related most closely to the type strains of (96.1 % similarity), (95.9 %), (95.8 %) and (95.5 %). The quinone system comprised menaquinone MK-9(H) as the major component and the polyamine pattern consisted of spermidine and spermine as major compounds. The predominant polar lipids were phosphatidylglycerol and unknown phospholipid PL3. Moderate amounts of diphosphatidylglycerol, an unknown glycolipid and three unknown phospholipids and minor amounts of an unknown phospholipid and a polar lipid were detected. The peptidoglycan type was A3′, based on -2,6-diaminopimelic acid with an interpeptide bridge consisting of a single glycine residue and a second glycine residue at position 1 of the peptide subunit. Peptidoglycan structure and major fatty acids (anteiso-C, iso-C and iso-C) supported the affiliation of strain CC-12602 to the genus . The results of physiological and biochemical tests allowed strain CC-12602 to be differentiated phenotypically from recognized species. Strain CC-12602 is therefore considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CC-12602 (=DSM 21800 =CCM 7685).

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2010-04-01
2020-06-05
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vol. , part 4, pp. 824 - 827

Polar lipid profile of CC-12602 after two-dimensional TLC and detection with molybdatophosphoric acid. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PL1–PL5, unknown phospholipids; GL, unknown glycolipid; L, unknown polar lipid (not stainable with any of the specific spray reagents applied, indicating that it does not contain a phosphate group, an amino group or a sugar moiety).



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