1887

Abstract

A Gram-negative, rod-shaped, halotolerant bacterium, designated strain CEE_131, which degraded high-molecular-mass polycyclic aromatic hydrocarbons of four and five rings, was isolated from intertidal sediment of Corcubion Ria in Cee, A Coruña, Spain. Direct sequencing showed ambiguities and suggested heterogeneity. Cloned 16S rRNA gene sequence PCR products yielded five different sequences varying at five positions. Strain CEE_131 showed rather distant relationships to its phylogenetically closest neighbours, including the genera and , exhibiting 91 % sequence similarity with BA131 and subsp. DSM 4597. The major fatty acids were C 7, C and C 7. The DNA G+C content was 41.7 mol%. On the basis of these distinct phenotypic and genotypic characteristics, strain CEE_131 is considered to represent a novel species in a new genus in the class , for which the name gen. nov., sp. nov. is proposed. The type strain is CEE_131 (=DSM 21945=CECT 7479).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013532-0
2010-03-01
2020-08-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/3/504.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013532-0&mimeType=html&fmt=ahah

References

  1. Bosshard P. P., Zbinden R., Altwegg M. 2002; Paenibacillus turicensis sp. nov., a novel bacterium harbouring heterogeneities between 16S rRNA genes. Int J Syst Evol Microbiol 52:2241–2249
    [Google Scholar]
  2. Brettar I., Christen R., Höfle M. G. 2002; Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52:1851–1857
    [Google Scholar]
  3. Brettar I., Christen R., Höfle M. G. 2006; Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56:2177–2183 [CrossRef]
    [Google Scholar]
  4. Bricker B. J. 2000; Characterization of the three ribosomal RNA operons rrnA, rrnB and rrnC , from Brucella melitensis . Gene 255:117–126 [CrossRef]
    [Google Scholar]
  5. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli . J Mol Biol 148:107–127 [CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  7. Cilia V., Lafay B., Christen R. 1996; Sequence heterogeneities among 16S ribosomal RNA sequences, and their effect on phylogenetic analysis at the species level. Mol Biol Evol 13:451–461
    [Google Scholar]
  8. Clayton R. A., Sutton G., Hinkle P. S. Jr, Bult C., Fields C. 1995; Intraspecific variation in small-subunit rRNA sequences in GenBank: why single species may not adequately represent prokaryotic taxa. Int J Syst Bacteriol 45:595–599 [CrossRef]
    [Google Scholar]
  9. Déziel E., Comeau Y., Villemur R. 1999; Two-liquid-phase bioreactors for enhanced degradation of hydrophobic/toxic compounds. Biodegradation 10:219–233 [CrossRef]
    [Google Scholar]
  10. Eisler R. 1987; Polycyclic Aromatic Hydrocarbons Hazards to Fish, Wildlife and Invertebrates: a Synoptic Review . Biol Report 85 (1.11: Washington, DC: US Fish and Wildlife Service;
    [Google Scholar]
  11. Galtier N., Gouy M., Gautier C. 1996; seaview and phylo_win, two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12:543–548
    [Google Scholar]
  12. Gauthier E., Déziel E., Villemur R., Juteau P., Lépine F., Beaudet R. 2003; Initial characterization of new bacteria degrading high-molecular-weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two-liquid-phase culture system. J Appl Microbiol 94:301–311 [CrossRef]
    [Google Scholar]
  13. Halpern M., Senderovich Y., Snir S. 2007; Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. Int J Syst Evol Microbiol 57:1872–1875 [CrossRef]
    [Google Scholar]
  14. Hughes J. B., Beckles D. M., Chandra S. D., Ward C. H. 1997; Utilization of bioremediation processes for the treatment of PAH-contaminated sediments. J Ind Microbiol Biotechnol 18:152–160
    [Google Scholar]
  15. Indarti E., Majid M. I. A., Hashim R., Chong A. 2005; Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil. J Food Compost Anal 18:161–170 [CrossRef]
    [Google Scholar]
  16. Juhasz A. L., Naidu R. 2000; Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene. Int Biodeterior Biodegrad 45:57–88 [CrossRef]
    [Google Scholar]
  17. Kennish M. J. 1992; Polynuclear aromatic hydrocarbons. In Ecology of Estuaries: Anthropogenic Effects pp 133–182 Boca Raton, FL: CRC Press;
    [Google Scholar]
  18. Kiyohara H., Nagao K., Yana K. 1982; Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl Environ Microbiol 43:454–457
    [Google Scholar]
  19. Liefting L. W., Andersen M. T., Beever R. E., Gardner R. C., Forster R. L. 1996; Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasm. Appl Environ Microbiol 62:3133–3139
    [Google Scholar]
  20. Martinez-Murcia A. J., Anton A. I., Rodriguez-Valera F. 1999; Patterns of sequence variation in two regions of the 16S rRNA genes multigene family of Escherichia coli . Int J Syst Bacteriol 49:601–610 [CrossRef]
    [Google Scholar]
  21. Merchant M. M., Welsh A. K., McLean R. J. C. 2007; Rheinheimera texasensis sp. nov., a halointolerant freshwater oligotroph. Int J Syst Evol Microbiol 57:2376–2380 [CrossRef]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  23. Ninet B., Monod M., Emler S., Pawlowski J., Metral C., Rohner P., Auckenthaler R., Hirschel B. 1996; Two different 16S rRNA genes in a mycobacterial strain. J Clin Microbiol 34:2531–2536
    [Google Scholar]
  24. Nübel U., Engelen B., Felske A., Snaidr J., Wieshuber A., Amann R. I., Ludwig W., Backhaus H. 1996; Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J Bacteriol 178:5636–5643
    [Google Scholar]
  25. Pettersson B., Bölske G., Thiaucourt F., Uhlén M., Johansson K. E. 1998; Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes. J Bacteriol 180:2350–2358
    [Google Scholar]
  26. Powers E. M. 1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61:3756–3758
    [Google Scholar]
  27. Reischl U., Feldmann K., Naumann L., Gaugler B. J., Ninet B., Hirschel B., Elmer S. 1998; 16S rRNA sequence diversity in Mycobacterium celatum strains caused by presence of two different copies of 16S rRNA gene. J Clin Microbiol 36:1761–1764
    [Google Scholar]
  28. Rodríguez-Blanco A., Ghiglione J. F., Catala P., Casamayor E. O., Lebaron P. 2009; Spatial comparison of total vs. active bacterial populations by coupling genetic fingerprinting and clone library analyses in the NW Mediterranean Sea. FEMS Microbiol Ecol 67:30–32
    [Google Scholar]
  29. Romanenko L. A., Uchino M., Falsen E., Zhukova N. V., Mikhailov V. V., Uchimura T. 2003; Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53:1973–1977 [CrossRef]
    [Google Scholar]
  30. Ryu S. H., Chung B. S., Park M., Lee S. S., Lee S.-S., Jeon C. O. 2008; Rheinheimera soli sp. nov., a gammaproteobacterium isolated from soil in Korea. Int J Syst Evol Microbiol 58:2271–2274
    [Google Scholar]
  31. Sohn J. H., Kwon K. K., Kang J. H., Jung H. B., Kim S. J. 2004; Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from sediment. Int J Syst Evol Microbiol 54:1483–1487 [CrossRef]
    [Google Scholar]
  32. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  33. Yoon J. H., Bae S. E., Kang S. J., Oh T. K. 2007; Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:1386–1390 [CrossRef]
    [Google Scholar]
  34. Zhang X., Sun L., Qiu F., McLean R. J. C., Jiang R., Song W. 2008; Rheinheimera tangshanensis sp. nov., a rice root-associated bacterium. Int J Syst Evol Microbiol 58:2420–2424 [CrossRef]
    [Google Scholar]
  35. ZoBell C. E. 1946 Marine Microbiology. a Monograph on Hydromicrobiology Waltham, MA: Chronica Botanica;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013532-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013532-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error