1887

Abstract

A Gram-negative, rod-shaped, halotolerant bacterium, designated strain CEE_131, which degraded high-molecular-mass polycyclic aromatic hydrocarbons of four and five rings, was isolated from intertidal sediment of Corcubion Ria in Cee, A Coruña, Spain. Direct sequencing showed ambiguities and suggested heterogeneity. Cloned 16S rRNA gene sequence PCR products yielded five different sequences varying at five positions. Strain CEE_131 showed rather distant relationships to its phylogenetically closest neighbours, including the genera and , exhibiting 91 % sequence similarity with BA131 and subsp. DSM 4597. The major fatty acids were C 7, C and C 7. The DNA G+C content was 41.7 mol%. On the basis of these distinct phenotypic and genotypic characteristics, strain CEE_131 is considered to represent a novel species in a new genus in the class , for which the name gen. nov., sp. nov. is proposed. The type strain is CEE_131 (=DSM 21945=CECT 7479).

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2010-03-01
2020-01-19
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Transmission electron micrograph obtained from sectioned cells of strain CEE_131 grown in marine broth for 12h at 25°C. Bar, 500nm.

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Effects of temperature (a), pH (b) and salinity (c) on growth of strain CEE_131 .

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