1887

Abstract

A Gram-negative motile rod, strain SAM4, was isolated from the highest positive dilution of a most probable number series inoculated with tidal-flat sediments from the German North Sea coast. The isolate grew at 4–35 °C and showed constant growth yields throughout almost the whole temperature range. Growth was observed between pH 6 and 9 and at salinities of 0.3–10.2 %. Strain SAM4 required Na for growth, contained bacteriochlorophyll and was catalase- and oxidase-positive. It was nutritionally versatile growing on a variety of carbon compounds including carbohydrates, amino acids and organic acids like lactate or succinate. It grew anaerobically on complex media such as marine broth, indicating fermentation, and by reducing trimethylammonium oxide. The dominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol, whereas only traces of phosphatidylcholine and an unidentified lipid were found. The major fatty acid was -C 7. The DNA G+C content was 56.4 mol%. The isolate was identified as a member of the clade within the class . However, based on phylogenetic, phenotypic and physiological data, it clearly differs from its closest relative . Therefore, a novel species is proposed: sp. nov., with strain SAM4 (=DSM 17270=LMG 23018) as the type strain. Emended descriptions of the genus and of are also presented.

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2010-08-01
2019-08-19
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vol. , part 8, pp. 1770 - 1777

Growth rate (a) and growth yield (b) of strain SAM4 against temperature. Analyses were performed in duplicate with the lines representing the mean.



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