The 16S rRNA genes of Ruegeria pelagia NBRC 102038T and Ruegeria mobilis NBRC 101030T were resequenced and the results confirmed that they differ by only one base in their almost full-length sequences (1425 nt). The gyrB gene sequence similarity between the two strains was also high (97.7 %). The outcome of API 20NE, API ZYM and antibiotic susceptibility tests showed that the two strains show only one difference, in β-galactosidase activity, in API tests and five differences in susceptibility among 30 tested antibiotics. In addition, similar BOX-PCR fingerprints were obtained and the DNA–DNA relatedness between the two strains was 91±4 %. On the basis of these results, it is concluded that Ruegeria pelagia Lee et al. 2007 is a later heterotypic synonym of Ruegeria mobilis Muramatsu et al. 2007.
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
(editors) 1995Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;
LaiQ.,
YuanJ.,
WuC.,
ShaoZ.2009; Oceanibaculum indicum gen. nov., sp. nov. isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737[CrossRef]
LiuC.,
ShaoZ.2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186[CrossRef]
MuramatsuY.,
UchinoY.,
KasaiH.,
SuzukiK.,
NakagawaY.2007; Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57:1304–1309[CrossRef]
VersalovicJ.,
KoeuthT.,
LupskiJ. R.1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
YamamotoS.,
KasaiH. L.,
ArnoldD. W.,
JacksonR.,
VivianA.,
HarayamaS.2000; Phylogeny of the genus Pseudomonas : intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146:2385–2394