1887

Abstract

The 16S rRNA genes of NBRC 102038 and NBRC 101030 were resequenced and the results confirmed that they differ by only one base in their almost full-length sequences (1425 nt). The gene sequence similarity between the two strains was also high (97.7 %). The outcome of API 20NE, API ZYM and antibiotic susceptibility tests showed that the two strains show only one difference, in -galactosidase activity, in API tests and five differences in susceptibility among 30 tested antibiotics. In addition, similar BOX-PCR fingerprints were obtained and the DNA–DNA relatedness between the two strains was 91±4 %. On the basis of these results, it is concluded that Lee 2007 is a later heterotypic synonym of Muramatsu 2007.

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2010-08-01
2021-04-19
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References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1995 Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology , 3rd edn. New York: Wiley;
    [Google Scholar]
  2. Dong X.-Z., Cai M.-Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation;
    [Google Scholar]
  3. Lai Q., Yuan J., Wu C., Shao Z. 2009; Oceanibaculum indicum gen. nov., sp. nov. isolated from deep seawater of the Indian Ocean. Int J Syst Evol Microbiol 59:1733–1737 [CrossRef]
    [Google Scholar]
  4. Lee K., Choo Y.-J., Giovannoni J. S., Cho J.-C. 2007; Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean. Int J Syst Evol Microbiol 57:1815–1818 [CrossRef]
    [Google Scholar]
  5. Liu C., Shao Z. 2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [CrossRef]
    [Google Scholar]
  6. Muramatsu Y., Uchino Y., Kasai H., Suzuki K., Nakagawa Y. 2007; Ruegeria mobilis sp. nov., a member of the Alphaproteobacteria isolated in Japan and Palau. Int J Syst Evol Microbiol 57:1304–1309 [CrossRef]
    [Google Scholar]
  7. Rzhetsky A., Nei M. 1992; A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 9:945–967
    [Google Scholar]
  8. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095
    [Google Scholar]
  9. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  10. Shieh W. Y., Chen Y.-W., Chaw S.-M., Chiu H.-H. 2003; Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53:479–484 [CrossRef]
    [Google Scholar]
  11. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  12. Vandecandelaere I., Nercessian O., Segaert E., Achouak W., Faimali M., Vandamme P. 2008; Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm. Int J Syst Evol Microbiol 58:2726–2733 [CrossRef]
    [Google Scholar]
  13. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  15. Yamamoto S., Kasai H. L., Arnold D. W., Jackson R., Vivian A., Harayama S. 2000; Phylogeny of the genus Pseudomonas : intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146:2385–2394
    [Google Scholar]
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