1887

Abstract

Four gamma- and UV-radiation-resistant bacterial strains, designated TDMA-24, TDMA-24-2, TDMA-24-3 and TDMA-24-4, were isolated from a fresh-water sample collected at Misasa, Tottori, Japan. Cells of these strains were Gram-reaction-positive, non-motile, non-spore-forming, rod-shaped and formed red colonies. The genomic DNA G+C contents ranged from 70.5 to 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus , the highest sequence similarities being with PB314 (98 %) and Ho-08 (97 %). The polar lipid profile of strain TDMA-24 comprised three unidentified phosphoglycolipids, five unidentified glycolipids and seven unidentified polar lipids. MK-8 was the predominant respiratory quinone. Major fatty acids were iso-C, Cω6, C, C and summed feature 3 (iso-C 2-OH and/or Cω7). On the basis of their phylogenetic positions and chemotaxonomic and phenotypic characteristics, the novel isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TDMA-24 ( = JCM 14369  = NBRC 102115  = CCUG 53609).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.013482-0
2011-06-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/6/1448.html?itemId=/content/journal/ijsem/10.1099/ijs.0.013482-0&mimeType=html&fmt=ahah

References

  1. Asker D. , Beppu T. , Ueda K. . ( 2007; a). Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan. . FEMS Microbiol Lett 273:, 140–148. [CrossRef].[PubMed]
    [Google Scholar]
  2. Asker D. , Beppu T. , Ueda K. . ( 2007; b). Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan. . Appl Microbiol Biotechnol 77:, 383–392. [CrossRef].[PubMed]
    [Google Scholar]
  3. Asker D. , Awad T. S. , Beppu T. , Ueda K. . ( 2008; ). Deinococcus misasensis and Deinococcus roseus, novel members of the genus Deinococcus, isolated from a radioactive site in Japan. . Syst Appl Microbiol 31:, 43–49. [CrossRef].[PubMed]
    [Google Scholar]
  4. Asker D. , Awad T. S. , Beppu T. , Ueda K. . ( 2009; ). Deinococcus aquiradiocola sp. nov., isolated from a radioactive site in Japan. . Int J Syst Evol Microbiol 59:, 144–149. [CrossRef].[PubMed]
    [Google Scholar]
  5. Barrow G. I. , Feltham R. K. A. . (editors) ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  6. Battista J. R. , Rainey F. A. . ( 2001; ). Order I. Deinococcales Rainey, Nobre, Schumann, Stackebrandt and da Costa 1997, 513vp . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 395–403. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . . New York:: Springer-Verlag;.
    [Google Scholar]
  7. Brooks B. W. , Murray R. G. E. . ( 1981; ). Nomenclature for “Micrococcus radiodurans” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. . Int J Syst Bacteriol 31:, 353–360. [CrossRef]
    [Google Scholar]
  8. Callegan R. P. , Nobre M. F. , McTernan P. M. , Battista J. R. , Navarro-González R. , McKay C. P. , da Costa M. S. , Rainey F. A. . ( 2008; ). Description of four novel psychrophilic, ionizing radiation-sensitive Deinococcus species from alpine environments. . Int J Syst Evol Microbiol 58:, 1252–1258. [CrossRef].[PubMed]
    [Google Scholar]
  9. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef].[PubMed]
    [Google Scholar]
  10. Collins M. D. . ( 1994; ). Isoprenoid quinones. . In Chemical Methods in Prokaryotic Systematics, pp. 265–310. Edited by O’Donnell M. G. A. G. . . Chichester:: Wiley;.
    [Google Scholar]
  11. de Groot A. , Chapon V. , Servant P. , Christen R. , Saux M. F. , Sommer S. , Heulin T. . ( 2005; ). Deinococcus deserti sp. nov., a gamma-radiation-tolerant bacterium isolated from the Sahara Desert. . Int J Syst Evol Microbiol 55:, 2441–2446. [CrossRef].[PubMed]
    [Google Scholar]
  12. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef].[PubMed]
    [Google Scholar]
  13. Embley T. M. , Odonnell A. G. , Wait R. , Rostron J. . ( 1987; ). Lipid and cell wall amino acid composition in the classification of members of the genus Deinococcus . . Syst Appl Microbiol 10:, 20–27.[CrossRef]
    [Google Scholar]
  14. Felsenstein J. . ( 1993; ). phylip (phylogeny inference package), version 3.5.. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  15. Ferreira A. C. , Nobre M. F. , Rainey F. A. , Silva M. T. , Wait R. , Burghardt J. , Chung A. P. , da Costa M. S. . ( 1997; ). Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. . Int J Syst Bacteriol 47:, 939–947. [CrossRef].[PubMed]
    [Google Scholar]
  16. Gertz E. M. . ( 2005; ). blast Scoring Parameters.. ftp://ftp.ncbi.nlm.nih.gov/blast/documents/developer/scoring.pdf.
  17. Hirsch P. , Gallikowski C. A. , Siebert J. , Peissl K. , Kroppenstedt R. , Schumann P. , Stackebrandt E. , Anderson R. . ( 2004; ). Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. . Syst Appl Microbiol 27:, 636–645. [CrossRef].[PubMed]
    [Google Scholar]
  18. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  19. Im W. T. , Jung H. M. , Ten L. N. , Kim M. K. , Bora N. , Goodfellow M. , Lim S. , Jung J. , Lee S. T. . ( 2008; ). Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 58:, 2348–2353. [CrossRef].[PubMed]
    [Google Scholar]
  20. Kämpfer P. , Lodders N. , Huber B. , Falsen E. , Busse H. J. . ( 2008; ). Deinococcus aquatilis sp. nov., isolated from water. . Int J Syst Evol Microbiol 58:, 2803–2806. [CrossRef].[PubMed]
    [Google Scholar]
  21. Lai W. A. , Kämpfer P. , Arun A. B. , Shen F. T. , Huber B. , Rekha P. D. , Young C. C. . ( 2006; ). Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L.. Int J Syst Evol Microbiol 56:, 787–791. [CrossRef].[PubMed]
    [Google Scholar]
  22. Mesbah M. , Whitman W. B. . ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef].[PubMed]
    [Google Scholar]
  23. O’Brien M. , Colwell R. . ( 1988; ). Characterization tests for numerical taxonomic studies. . Methods Microbiol 19:, 69–104. [CrossRef]
    [Google Scholar]
  24. Perrière G. , Gouy M. . ( 1996; ). www-query: an on-line retrieval system for biological sequence banks. . Biochimie 78:, 364–369. [CrossRef].[PubMed]
    [Google Scholar]
  25. Rainey F. A. , Nobre M. F. , Schumann P. , Stackebrandt E. , da Costa M. S. . ( 1997; ). Phylogenetic diversity of the deinococci as determined by 16S ribosomal DNA sequence comparison. . Int J Syst Bacteriol 47:, 510–514. [CrossRef].[PubMed]
    [Google Scholar]
  26. Rainey F. A. , Ray K. , Ferreira M. , Gatz B. Z. , Nobre M. F. , Bagaley D. , Rash B. A. , Park M. J. , Earl A. M. et al. ( 2005; ). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. . Appl Environ Microbiol 71:, 5225–5235. [CrossRef].[PubMed]
    [Google Scholar]
  27. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Schleifer K. H. , Kandler O. . ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  29. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  30. Suresh K. , Reddy G. S. , Sengupta S. , Shivaji S. . ( 2004; ). Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. . Int J Syst Evol Microbiol 54:, 457–461. [CrossRef].[PubMed]
    [Google Scholar]
  31. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef].[PubMed]
    [Google Scholar]
  32. Tindall B. J. . ( 1990; ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66:, 199–202. [CrossRef]
    [Google Scholar]
  33. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  34. Zhang Y. Q. , Sun C. H. , Li W. J. , Yu L. Y. , Zhou J. Q. , Zhang Y. Q. , Xu L. H. , Jiang C. L. . ( 2007; ). Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. . Int J Syst Evol Microbiol 57:, 370–375. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.013482-0
Loading
/content/journal/ijsem/10.1099/ijs.0.013482-0
Loading

Data & Media loading...

Supplements

[PDF] 

PDF

vol. , part 6, pp. 1448 - 1453

IMAGE

vol. , part 6, pp. 1448 - 1453

IMAGE

vol. , part 6, pp. 1448 - 1453

IMAGE

[PDF] 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error