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Abstract

Two aerobic, Gram-reaction-negative, non-spore-forming bacterial strains, 4M29 and 4M40, were isolated from cotton composts. The two strains grew in the presence of 0–5 % (w/v) NaCl (optimum growth in the absence of NaCl), at pH 6.0–8.0 (optimum, pH 7.0) and at 15–45 °C (optimum, 30 °C). The strains shared 97.1 % 16S rRNA gene sequence similarity. Strains 4M29 and 4M40 showed the next highest levels of 16S rRNA gene sequence similarity to Jip14 (95.6 and 94.4 %, respectively) and DCY14 (95.2 and 93.8 %). The level of DNA–DNA relatedness between strains 4M29 and 4M40 was 38 %. The two strains contained iso-C, summed feature 3 (comprising iso-C 2-OH and/or C 7) and iso-C 3-OH as major fatty acids, MK-7 as the major respiratory quinone, homospermidine as the only polyamine and phosphatidylethanolamine as the major polar lipid. The DNA G+C contents of strains 4M29 and 4M40 were 47.6 and 48.6 mol%, respectively. On the basis of phylogenetic and phenotypic data, strains 4M29 and 4M40 are considered to represent two novel species of the genus , for which the names sp. nov. (type strain 4M29 =KACC 10955 =JCM 15977) and sp. nov. (type strain 4M40 =KACC 10972 =JCM 15978) are proposed.

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2010-08-01
2019-12-05
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vol. , part 8, pp. 1849 - 1853

Two-dimensional thin-layer chromatograms showing the polar lipid profiles of Jip14 (a) and strains 4M29 (b) and 4M40 (c). PE, Phosphatidylethanolamine; SL, sphingolipid; AL, unknown aminolipid; PN, unknown aminophospholipid; PL, unknown phospholipid; L, unknown lipid.



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