An anoxygenic, phototrophic gammaproteobacterium designated strain JA418 was isolated from a sediment sample collected from the Baitarani River, Orissa, India. The bacterium was a Gram-negative, motile rod with a single polar flagellum. Bacteriochlorophyll and rhodopin were the major photosynthetic pigments. The organism grew best at slightly alkaline pH (8–8.5) and lacked chemotrophic growth. The major fatty acids were C, C 7/C 6 and C 7. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strain JA418 clusters with species of the genus belonging to the class . The highest 16S rRNA gene sequence similarities of strain JA418 were found with the type strains of (95.9 %), (95.6 %), (95.5 %) and (95.6 %). The DNA base composition of strain JA418 was 71.4 mol% G+C (by HPLC). Based on the 16S rRNA gene sequence analysis and physiological and chemotaxonomic characteristics, strain JA418 is sufficiently different from other species to merit the description of a novel species, sp. nov., to accommodate it. The type strain is JA418 (=KCTC 5717 =NBRC 105221).


Article metrics loading...

Loading full text...

Full text loading...



  1. Aino, K., Hirota, K., Matsuno, T., Morita, N., Nodasaka, Y., Fujiwara, T., Matsuyama, H., Yoshimune, K. & Yumoto, I. ( 2008; ). Bacillus polygoni sp. nov., a moderately halophilic, non-motile obligate alkaliphile isolated from indigo balls. Int J Syst Evol Microbiol 58, 120–124.[CrossRef]
    [Google Scholar]
  2. Anil Kumar, P., Sasi Jyothsna, T. S., Srinivas, T. N. R., Sasikala, Ch., Ramana, Ch. V. & Imhoff, J. F. ( 2007; ). Marichromatium bheemlicum sp. nov., a non-diazotrophic, photosynthetic gammaproteobacterium from a marine aquaculture pond. Int J Syst Evol Microbiol 57, 1261–1265.[CrossRef]
    [Google Scholar]
  3. Arunasri, K., Sasikala, C., Ramana, C. V., Süling, J. & Imhoff, J. F. ( 2005; ). Marichromatium indicum sp. nov., a novel purple sulfur gammaproteobacterium from mangrove soil of Goa, India. Int J Syst Evol Microbiol 55, 673–679.[CrossRef]
    [Google Scholar]
  4. Imhoff, J. F. ( 2005; ). Family I. Chromatiaceae Bavendamm 1924, 125AL emend. Imhoff 1984b, 339. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 3–9. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  5. Imhoff, J. F., Süling, J. & Petri, R. ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef]
    [Google Scholar]
  6. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  7. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  8. Pfennig, N. & Trüper, H. G. ( 1992; ). The family Chromatiaceae. In The Prokaryotes. A Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3200–3221. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. Berlin, Heidelberg & New York: Springer.
  9. Sanger, F., Nicklen, S. & Coulson, A. R. ( 1977; ). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci U S A 74, 5463–5467.[CrossRef]
    [Google Scholar]
  10. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  11. Serrano, W., Amann, R. & Fischer, U. ( 2009; ). A new moderately thermophilic and high sulfide tolerant biotype of Marichromatium gracile, isolated from tidal sediments of German Wadden Sea: Marichromatium gracile biotype thermosulfidiphilum. Syst Appl Microbiol 32, 1–7.[CrossRef]
    [Google Scholar]
  12. Takaichi, S. & Shimada, K. ( 1992; ). Characterization of carotenoids in photosynthetic bacteria. Methods Enzymol 213, 374–385.
    [Google Scholar]
  13. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]

Data & Media loading...


vol. , part 5, pp. 1103 - 1107

Effects of NaCl concentration and pH on growth of strain JA418 .

Whole-cell absorption spectrum of strain JA418 and absorption spectrum of pigments of strain JA418 extracted with acetone.

[PDF file of Supplementary Figs S1 and S2](99 KB)


Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error