1887

Abstract

Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the strains were located in the broad cluster of the genus . Chemotaxonomic data [cell-wall diamino acid, -diaminopimelic acid; cell-wall peptidoglycan, type III (A1); major sugars, galactose and arabinose; major menaquinone, MK-9(H); major fatty acid, iso-C; major phospholipid, phosphatidylethanolamine] supported the affiliation of all six strains to the genus . The results of DNA–DNA hybridization with DNA from type strains of species with validly published names revealed three DNA–DNA relatedness groups. Group I (ID03-0561) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784, ID03-0808 and ID03-0809) formed a group with high relatedness (98–100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810 and ID03-0813) showed 58–68 % relatedness to NBRC 15668 and showed low relatedness (2–24 %) to the other strains studied. The description of three novel species is proposed: sp. nov., for the single strain in group I (type strain ID03-0561 =BTCC B-554 =NBRC 104211), sp. nov., for the strains in group II (type strain ID03-0784 =BTCC B-555 =NBRC 104212), and sp. nov., for the strains in group III (type strain ID03-0810 =BTCC B-558 =NBRC 105526).

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2010-10-01
2021-10-16
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