RT Journal Article SR Electronic(1) A1 Echigo, Akinobu A1 Minegishi, Hiroaki A1 Mizuki, Toru A1 Kamekura, Masahiro A1 Usami, RonYR 2010 T1 Geomicrobium halophilum gen. nov., sp. nov., a moderately halophilic and alkaliphilic bacterium isolated from soil JF International Journal of Systematic and Evolutionary Microbiology, VO 60 IS 4 SP 990 OP 995 DO https://doi.org/10.1099/ijs.0.013268-0 PB Microbiology Society, SN 1466-5034, AB Two moderately halophilic and alkaliphilic bacteria, designated strains BH1T and HN5, were isolated from forest soil and garden soil, respectively, in Japan. Cells of strains BH1T and HN5 were non-motile, aerobic, bean-shaped, formed irregular clusters with 2–20 cells, Gram-positive and contained A1γ, meso-diaminopimelic acid-type murein. Spore formation was not detected. Growth occurred in 5–25 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 6.0–10.0 (optimum, pH 8.5–9.0) and at 20–40 °C (optimum, 30 °C). The predominant isoprenoid quinones were menaquinone-7 and menaquinone-6. The phospholipids were diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were i-C15 : 0, i-C17 : 0 and i-C18 : 0. The DNA G+C content of strains BH1T and HN5 was 45 and 46 mol%, respectively. The 16S rRNA gene sequences of the two strains were 99.9 % similar. DNA–DNA hybridization results indicated high levels of relatedness (88 and 85 % reciprocally). Similarities with recognized species were less than 90.2 %. The phylogenetic and phenotypic characteristics indicated that strains BH1T and HN5 represent a novel species in a new genus, for which the name Geomicrobium halophilum gen. nov., sp. nov. is proposed. The type strain is BH1T (=JCM 15647T=DSM 21769T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.013268-0