1887

Abstract

gen. nov., sp. nov. is proposed to accommodate three strains of slowly growing, chemo-organotrophic bacteria isolated from acidic peat bogs. These bacteria were strictly aerobic, Gram-negative, colourless, non-motile coccoids or short rods that multiplied by normal cell division and formed irregularly shaped cell aggregates. Strains MPL3, MPL1011 and MOB76 were acidotolerant, mesophilic organisms capable of growth at pH 4.5–7.2 and between 4 and 33 °C (optimum growth at pH 5.5–6.5 and 22–28 °C). The preferred growth substrates were sugars, some heteropolysaccharides and galacturonic and glucuronic acids, which are released during decomposition of moss. The major fatty acids were iso-C, C and summed feature 3 (iso-C 2-OH and/or C 7); the major quinones were MK-9 and MK-10. The DNA G+C content was 55.5–56.5 mol%. Strains MPL3, MPL1011 and MOB76 possessed nearly identical 16S rRNA gene sequences and belonged to the phylum . They represent the first taxonomically characterized members of acidobacterial subdivision 3 and display only 81.7–86.7 % 16S rRNA gene sequence similarity to other members of the with validly published names. Therefore, strains MPL3, MPL1011 and MOB76 are classified as representatives of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; strain MPL3 (=ATCC BAA-1390 =DSM 18758) is the type strain of .

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2010-02-01
2024-03-29
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References

  1. Barns S. M., Takala S. L., Kuske C. R. 1999; Wide distribution and diversity of members of the bacterial kingdom Acidobacterium in the environment. Appl Environ Microbiol 65:1731–1737
    [Google Scholar]
  2. Clymo R. S. 1963; Ion exchange in Sphagnum and its relation to bog ecology. Ann Bot (Lond) 27:309–324
    [Google Scholar]
  3. Clymo R. S. 1964; The origin of acidity in Sphagnum bogs. Bryologist 67:427–431 [CrossRef]
    [Google Scholar]
  4. Coates J. D., Ellis D. J., Gaw C. V., Lovley D. R. 1999; Geothrix fermentans gen. nov., sp. nov. a novel Fe(III)-reducing bacterium from a hydrocarbon-contaminated aquifer. Int J Syst Bacteriol 49:1615–1622 [CrossRef]
    [Google Scholar]
  5. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366
    [Google Scholar]
  6. Davis K. E. R., Joseph S. J., Janssen P. H. 2005; Effects of growth medium, inoculum size, and incubation time on culturability and isolation of soil bacteria. Appl Environ Microbiol 71:826–834 [CrossRef]
    [Google Scholar]
  7. Dedysh S. N., Pankratov T. A., Belova S. E., Kulichevskaya I. S., Liesack W. 2006; Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog. Appl Environ Microbiol 72:2110–2117 [CrossRef]
    [Google Scholar]
  8. Eichorst S. A., Breznak J. A., Schmidt T. M. 2007; Isolation and characterization of soil bacteria that define Terriglobus gen. nov., in the phylum Acidobacteria . Appl Environ Microbiol 732708–2717 [CrossRef]
    [Google Scholar]
  9. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  10. Fukunaga Y., Kurahashi M., Yanagi K., Yokota A., Harayama S. 2008; Acanthopleuribacter pedis gen. nov., a marine bacterium isolated from a chiton, and description of Acanthopleuribacteraceae fam.nov., Acanthopleuribacterales ord. nov., Holophagaceae fam. nov., Holophagales ord. nov. and Holophagae classis nov. in the phylum ‘ Acidobacteria ’. Int J Syst Evol Microbiol 58:2597–2601 [CrossRef]
    [Google Scholar]
  11. Hugenholtz P., Goebel B. M., Pace N. R. 1998; Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 180:4765–4774
    [Google Scholar]
  12. Janssen P. H. 2006; Identifying the dominant soil bacterial taxa in libraries of 16S rRNA and 16S rRNA genes. Appl Environ Microbiol 72:1719–1728 [CrossRef]
    [Google Scholar]
  13. Janssen P. H., Yates P. S., Grinton B. E., Taylor P. M., Sait M. 2002; Improved culturability of soil bacteria and isolation in pure culture of novel members of the divisions Acidobacteria , Actinobacteria , Proteobacteria , and Verrucomicrobia . Appl Environ Microbiol 68:2391–2396 [CrossRef]
    [Google Scholar]
  14. Joseph S. J., Hugenholtz P., Sangwan P., Osborne C. A., Janssen P. H. 2003; Laboratory cultivation of widespread and previously uncultured soil bacteria. Appl Environ Microbiol 69:7210–7215 [CrossRef]
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  16. Kishimoto N., Kosako Y., Tano T. 1991; Acidobacterium capsulatum gen. nov., sp. nov.: an acidophilic chemoorganotrophic bacterium containing menaquinone from acidic mineral environment. Curr Microbiol 22:1–7 [CrossRef]
    [Google Scholar]
  17. Koch I. H., Gich F., Dunfield P. F., Overmann J. 2008; Edaphobacter modestus gen. nov., sp. nov., and Edaphobacter aggregans sp. nov., acidobacteria isolated from alpine and forest soils. Int J Syst Evol Microbiol 58:1114–1122 [CrossRef]
    [Google Scholar]
  18. Liesack W., Bak F., Kreft J. U., Stackebrandt E. 1994; Holophaga foetida gen. nov., sp. nov. a new, homoacetogenic bacterium degrading methoxylated aromatic compounds. Arch Microbiol 162:85–90
    [Google Scholar]
  19. Ludwig W., Bauer S. H., Bauer M., Held I., Kirchhof G., Schulze R., Huber I., Spring S., Hartmann A., Schleifer K. H. 1997; Detection and in situ identification of representatives of a widely distributed new bacterial phylum. FEMS Microbiol Lett 153:181–190 [CrossRef]
    [Google Scholar]
  20. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi G. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  21. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  22. Pankratov T. A., Serkebaeva Y. M., Kulichevskaya I. S., Liesack W., Dedysh S. N. 2008; Substrate-induced growth and isolation of Acidobacteria from acidic Sphagnum peat. ISME J 2:551–560 [CrossRef]
    [Google Scholar]
  23. Sait M., Hugenholtz P., Janssen P. H. 2002; Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. Environ Microbiol 4:654–666 [CrossRef]
    [Google Scholar]
  24. Staley J. T., Fuerst J. A., Giovannoni S., Schlesner H. 1992; The order Planctomycetales and the genera Planctomyces , Pirellula , Gemmata , and Isosphaera . In The Prokaryotes: a Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp 3710–3731 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  25. Stott M. B., Crowe M. A., Mountain B. W., Smirnova A. V., Hou S., Alam M., Dunfield P. F. 2008; Isolation of novel bacteria, including a candidate division, from geothermal soils in New Zealand. Environ Microbiol 10:2030–2041 [CrossRef]
    [Google Scholar]
  26. Ward N. L., Challacombe J. F., Janssen P. H., Henrissat B., Coutinho P. M., Wu M., Xie G., Haft D. H., Sait M. other authors 2009; Three genomes from the phylum Acidobacteria provide insight into the lifestyles of these microorganisms in soils. Appl Environ Microbiol 75:2046–2056 [CrossRef]
    [Google Scholar]
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