1887

Abstract

A novel actinomycete, strain TVU1, was isolated from leaves of the indigenous South African plant . Applying a polyphasic approach, the isolate was identified as a member of the genus . Phylogenetic analysis of the 16S rRNA gene sequence showed that strain TVU1 was most closely related to DSM 43816. However, phylogenetic analysis based on gene sequences showed that strain TVU1 was most closely related to the type strains of and . DNA–DNA relatedness values between strain TVU1 and the type strains of , and were 7.6±4.5, 45.9±2.0 and 60.9±4.5 %, respectively. Strain TVU1 could be distinguished from the type strains of all three of these species by several physiological characteristics, such as colony colour, NaCl tolerance, growth temperature range and sole carbon source utilization pattern. Strain TVU1 (=DSM 45142=NRRL B-24576) therefore represents a novel species for which the name sp. nov. is proposed.

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2010-06-01
2021-02-28
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Atlas R. M. 1993 Handbook of Microbiological Media Edited by Parks L. C. Boca Raton, FL: CRC Press;
    [Google Scholar]
  3. Cook A. E., Meyers P. R. 2003; Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 53:1907–1915 [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Demain A. L., Davies J. E. (editors) 1999 Manual of Industrial Microbiology and Biotechnology , 2nd edn. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Everest G. J., Meyers P. R. 2009; The use of gyrB sequence analysis in the phylogeny of the genus Amycolatopsis . Antonie van Leeuwenhoek 95:1–11
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  8. Garcia L. C., Martínez-Molina E., Trujillo M. E. 2010; Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . Int J Syst Evol Microbiol 60:331–337 [CrossRef]
    [Google Scholar]
  9. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  10. Hirsch P., Mevs U., Kroppenstedt R. M., Schumann P., Stackebrandt E. 2004; Cryptoendolithic actinomycetes from Antarctic sandstone rock samples: Micromonospora endolithica sp. nov. and two isolates related to Micromonospora coerulea Jensen 1932. Syst Appl Microbiol 27:166–174 [CrossRef]
    [Google Scholar]
  11. Horan A. C., Brodsky B. C. 1986; Micromonospora rosaria sp. nov., nom. rev., the rosaramicin producer. Int J Syst Bacteriol 36:478–480 [CrossRef]
    [Google Scholar]
  12. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from denaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  13. Joffe P. 1993 The Gardener's Guide to South African Plants Cape Town; South Africa: Tafelberg;
    [Google Scholar]
  14. Kasai H., Tamura T., Harayama S. 2000; Intrageneric relationships among Micromonospora species deduced from gyrB -based phylogeny and DNA relatedness. Int J Syst Evol Microbiol 50:127–134 [CrossRef]
    [Google Scholar]
  15. Kawamoto I. 1989; Genus Micromonospora Ørskov 1923, 147AL . In Bergey's Manual of Systematic Bacteriology vol 4 pp 2442–2450 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  17. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  18. le Roes M., Goodwin C. M., Meyers P. R. 2008; Gordonia lacunae sp. nov., isolated from an estuary. Syst Appl Microbiol 31:17–23 [CrossRef]
    [Google Scholar]
  19. Manning J. 2003 Photographic Guide to the Wildflowers of South Africa Pretoria, South Africa: Briza Publications;
    [Google Scholar]
  20. Okazaki T. 2003; Studies on actinomycetes isolated from plant leaves. In Selective Isolation of Rare Actinomycetes , chapter 4 Edited by Kurtböke I. Queensland, Australia: University of the Sunshine Coast;
    [Google Scholar]
  21. Ørskov J. 1923 Investigations into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard;
    [Google Scholar]
  22. Rzhetsky A., Nei M. 1993; Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10:1073–1095
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Shen F.-T., Lu H.-L., Lin J.-L., Huang W.-S., Arun A. B., Young C.-C. 2006; Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene. Res Microbiol 157:367–375 [CrossRef]
    [Google Scholar]
  25. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  27. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. 2004; Micromonospora aurantionigra sp. nov., isolated from a peat swamp forest in Thailand. Actinomycetologica 18:8–14 [CrossRef]
    [Google Scholar]
  28. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. 2005; Micromonospora siamensis sp. nov., isolated from Thai peat swamp forest. J Gen Appl Microbiol 51:229–234 [CrossRef]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  30. Trujillo M. E., Fernández-Molinero C., Velázquez E., Kroppenstedt R. M., Schumann P., Mateos P. F., Martínez-Molina E. 2005; Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55:877–880 [CrossRef]
    [Google Scholar]
  31. Trujillo M. E., Kroppenstedt R. M., Schumann P., Carro L., Martínez-Molina E. 2006; Micromonospora coriariae sp. nov., isolated from root nodules of Coriaria myrtifolia . Int J Syst Evol Microbiol 56:2381–2385 [CrossRef]
    [Google Scholar]
  32. Trujillo M. E., Kroppenstedt R. M., Fernández-Molinero C., Schumann P., Martínez-Molina E. 2007; Micromonospora lupini sp. nov., and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius . Int J Syst Evol Microbiol 57:2799–2804 [CrossRef]
    [Google Scholar]
  33. Van Wyk B.-E., Van Oudtshoorn B., Gericke N. 1997 Medicinal Plants of South Africa. Pretoria South Africa: Briza Publications;
    [Google Scholar]
  34. Wagman G. H., Weinstein M. J. 1980; Antibiotic from Micromonospora . Annu Rev Microbiol 34:537–557 [CrossRef]
    [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  36. Wood S. A., Kirby B. M., Goodwin C. M., Le Roes M., Meyers P. R. 2007; PCR screening reveals unexpected antibiotic biosynthetic potential in Amycolatopsis sp. strain UM16. J Appl Microbiol 102:245–253 [CrossRef]
    [Google Scholar]
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